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PCR: TGM2 flox
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Export PDF
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| ID:
128
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Amplifluor:
No
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| Nucleotide sequence (5´ ⇒ 3´) |
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| Primer name |
Sequence |
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P2
|
CAGATAGGGATACAAGAAGCATTGAAG
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P3
|
GCCCCACAAAGGAGCAAGTGTTACTATGTC
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| Reagents / Constituents |
Commercial name |
Stock
Concentration |
20x Primer mix
concentration (μM) |
Volume (μL) |
| DNA Sample |
|
NA ng/μl
|
|
0.5
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| 10x Buffer |
5x GoTaq
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7.5 mM MgCl2
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|
4.0
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| dNTPs |
|
2.0 mM
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|
0.4
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| 20x amplifluor SNP FAM Primer |
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NA μM
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NA
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| 20x amplifluor SNP JOE Primer |
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NA μM
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NA
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| Primer:
P2
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|
10.0 μM
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NA
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2.0
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| Primer:
P3
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|
10.0 μM
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NA
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2.0
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| Amplifluor Primer Volume |
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NA
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| Water |
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14.6
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| Taq Polyermase |
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0.1
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| Comments on protocol: |
5x GoTaq buffer is supplied by promega
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| Steps |
Temp (°C ) |
Time (seconds) |
| Hot start:
true
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| Initiation/Melting |
94.0
|
120
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| Denaturation |
94.0
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30
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| Annealing |
57.0
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30
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| Elongation |
72.0
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30
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Number of cycles
(repeat denature, anneal & elongate):
30
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| Strand completion (i.e. 72°C, 10 min) |
72.0
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360
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| Finish (i.e. 4°C, indefinite) |
4.0
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| % Agarose |
3.0
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| Volt |
100.0
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| Estimated Running Time (min) |
40.0
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| Picture |
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| Number |
Band (bp) |
Genotype |
| 1. |
100
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WT
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| 2. |
140
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FLOXED
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| 3. |
100 / 140
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WT/FLOXED
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