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PCR: Funky Genotyping
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Export PDF
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| ID:
387
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Amplifluor:
No
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| Nucleotide sequence (5´ ⇒ 3´) |
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| Primer name |
Sequence |
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Ndufs4exon3F2
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taggaagggagagacgagca
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Ndufs4exon3R2
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GATGCCCAACCCATCAAAG
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Ndufs4B2F2R
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TTACCCACTGAGCCATCTCAC
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| Reagents / Constituents |
Commercial name |
Stock
Concentration |
20x Primer mix
concentration (μM) |
Volume (μL) |
| DNA Sample |
|
NA ng/μl
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NA
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| 10x Buffer |
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NA mM MgCl2
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NA
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| dNTPs |
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NA mM
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NA
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| 20x amplifluor SNP FAM Primer |
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NA μM
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NA
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| 20x amplifluor SNP JOE Primer |
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NA μM
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NA
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| Primer:
Ndufs4exon3F2
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NA μM
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NA
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NA
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| Primer:
Ndufs4exon3R2
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NA μM
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NA
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NA
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| Primer:
Ndufs4B2F2R
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NA μM
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NA
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NA
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| Amplifluor Primer Volume |
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NA
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| Water |
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NA
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| Taq Polyermase |
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NA
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| Steps |
Temp (°C ) |
Time (seconds) |
| Hot start:
false
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| Initiation/Melting |
NA
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| Denaturation |
NA
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| Annealing |
NA
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| Elongation |
NA
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Number of cycles
(repeat denature, anneal & elongate):
NA
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| Strand completion (i.e. 72°C, 10 min) |
NA
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| Finish (i.e. 4°C, indefinite) |
NA
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| Number |
Band (bp) |
Genotype |
| 1. |
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