Home - Australian Phenome Bank

Mouse Strain information

APB ID

10330

Added

09/05/2023

Last Edited

26/11/2025

Strain nomenclature:

C57Bl/6N-OsxCrep53fl/flpRbfl/flR26Adar1E861A/Apb

Genetics

Genetic Details

Transgenic
CRISPR targeted
ES cells targeting (Homologous recombination)

ES Cell-Line Used

for p53 - Jos Jonkers floxed allele; for pRb - Tyler Jacks floxed allele (both previously submitted to PA for cryopreservation)

Mode of Inheritance

Semi-dominant

1. Affected Gene Name: Osx-Cre p53fl/fl pRbfl/fl R26-Adar1E861A

Chromosome	Unknown
Allele Name	Osx-Cre p53fl/fl pRbfl/fl R26-Adar1E861A

1. Transgene: Osx-Cre

CRISPR types

Insertion/Tagging

Point mutation sequence:

Insertion/Tagging details:

Lox-stop-lox Adar1 ires-GFP

Insertion/Tagging details:

The murine Adar1 cDNA were cloned from the pLVX-IRES-Puro cloned into the pR26-CAG/GFP-Asc vector. pR26 CAG/GFP Asc was a gift from Ralf Kuehn (Addgene plasmid # 74285; http://n2t.net/addgene:74285; RRID:Addgene_74285). All targeting constructs also included an Adar1 cDNA specific unique restriction site between the Adar1 cDNA and IRES-GFP could be used to confirm the cDNA inserted if required on genomic DNA PCR products. Rosa26-LoxStop-Lox-Adar1 mice were generated using CRISPR/Cas9 targeting in C57BL/6 zygotes by The Melbourne Advanced Genome Editing Center (MAGEC) at the Walter and Eliza Hall Institute (Parkville, Australia). All constructs were confirmed by Sanger sequencing and then after successful targeting by long range PCR, unique restriction digest and Sanger sequencing of the region in both the founders and subsequent generations. Adar1p150 is the isoform of ADAR1 expressed in the cytoplasm.

Guide(s) RNA sequence:

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Homozygous viable and fertile; stock maintained as Cre negative line with all other alleles homozygous. When crossed to Osx-Cre will develop osteosarcoma

Phenotype Heterozygous State

Heterozygous viable and fertile; stock maintained as Cre negative line with all other alleles homozygous. When crossed to Osx-Cre will develop osteosarcoma

Genetic Background Currently Maintained

C57Bl/6N

Strain identification

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Is the strain available in any form to other researchers

Possibly

APB stock stored as:

Cryopreserved sperm (from 3 mice)

Are live mice available?

Unknown

MTA needs to be signed?

Yes