Mouse Strain information
APB ID 10351
Added 20/07/2023
Last Edited 01/12/2023
Strain nomenclature: C57Bl/6-TFF2(CreERT2)-Rosa26-delta(D3muc)/Apb
Genetics
Strain Types Inbred
Genetic Details ES cells targeting (Homologous recombination)
ES Cell-Line Used tamoxifen inducible Cre knocked in to TFF2 locus X Rosa26 stop flox Winnie D3
Mode of Inheritance Semi-dominant
1.  Affected Gene Name: trefoil factor 2 (spasmolytic protein 1)  
Chromosome 17
Affected Gene Symbol Tff2
Genetic alteration of gene: To create the TFF2-CreERT2 mouse, we used the tamoxifen-inducible Cre transgene, CreERT2 (as modified by Tyler Jacks, Addgene). A knock in allele was constructed targeting CreERT2 to the last exon of TFF2, immediately downstream of the termination codon. Neo flanked by Frt sites was inserted between intron 3 and 4 for removal by Flp-mediated recombination. These mice crossed to Rosa26Winnie (∆D3): a knock in allele was constructed from the 1245bp Muc2 N-terminal D3 domain from Winnie (∆D3; Heazlewood, 2008) and placed adjacent to a floxed STOP cassette inside ROSA26 homology arms.
Location on chromosome (bp): 31360036-31363256 bp
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

 MGI:1306805
Allele Name TFF2
Ensembl Gene ID: ENSMUSG00000024028
Point mutation sequence:
Insertion/Tagging details:
Guide(s) RNA sequence:
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Normal
Phenotype Heterozygous State Normal
Genetic Background Currently Maintained C57Bl/6J
Strain identification
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
General information
Associated IP rights? Unknown
Does it Model a Human Condition? Unknown
Is the strain available in any form to other researchers Possibly
APB stock stored as: Cryopreserved sperm (from 3 mice)
Are live mice available? Unknown
MTA needs to be signed? Yes


     

 


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