Home - Australian Phenome Bank

Mouse Strain information

APB ID

1526

Added

05/07/2007

Last Edited

11/05/2014

Strain nomenclature:

B6.129P2-Psmb9tm1Stl/AnuApb

Nickname:

B6.LMP2-/-

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted knockout

Mode of Inheritance

Recessive

1. Affected Gene Name: proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2)

Chromosome	17
Affected Gene Symbol	Psmb9
Protein expression of altered gene:	Nil
Genetic alteration of gene:	A 0.8-kb region, containing a 3' portion of exon 2, was replaced by a neomycin selection cassette. Western analysis of extracts from homozygous mutant mice showed that the encoded protein was absent and that Psmb8 protein levels were unaffected.
Location on chromosome (bp):	34319044-34324275 bp, - strand From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:1346526
Synonyms	Lmp-2, Lmp2
Allele Name	targeted mutation 1, Susumu Tonegawa
Allele Symbol	Psmb9tm1Stl
MGI Allele Accession ID	MGI:2152729

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Mice homozygous for disruptions in this gene have a grossly normal phenotype but suffer from increased susceptibility to some viruses and have an increased risk of tumor development. Decreased CD8-positive T cell number: * by 36% in blood * by 22% in spleen * by 49% in thymus Abnormal CD8-positive, alpha-beta cytotoxic T cell morphology : * 5-6 fold lower levels of cytotoxic T lymphocyte precursors after infection with influenza A virus but not Sendai virus Abnormal immune system physiology: * splenic lymphoblasts and peritoneal exudates cells are less efficient stimulators of CD8+ T cells. Increased susceptibility to viral infection: * 5-6 fold lower levels of cytotoxic T lymphocyte precursors after infection with influenza A virus but not Sendai virus.

Genetic Background Currently Maintained

C57BL/6 x 129S2

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation
Haematology
Cancer

Keywords

T cell, CD8
tumour
viral infection
cytotoxic

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

MTA needs to be signed?

Yes