Home - Australian Phenome Bank

Mouse Strain information

APB ID

1626

Added

16/07/2007

Last Edited

11/05/2014

Strain nomenclature:

129X1;129S1-Cited2tm1.1Dunw/Apb

Nickname:

C2X1f

Strain designation name:

129X1;129S1-Cited2tm1.1Dunw/Apb

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted reporter
Targeted floxed

ES Cell-Line Used

W9.5

Mode of Inheritance

Recessive

1. Affected Gene Name: Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2

Chromosome	10
Affected Gene Symbol	Cited2
Protein expression of altered gene:	Normal
Genetic alteration of gene:	The neomycin resistance cassette was removed from Cited2tm1Dunw, leaving exon 2 floxed and a lacZ downstream of the exon.
Location on chromosome (bp):	17443032-17444750 bp, + strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:1306784
Synonyms	ER154-like, Mrg1, Msg2, p35srj
Allele Name	targeted mutation 1.1, Sally L Dunwoodie
Allele Symbol	Cited2tm1.1Dunw
MGI Allele Accession ID	MGI:3696062

Mutant Construction Technique

CRE recombinase-loxP technology

Candidate Gene

Cited2

Phenotype

Phenotype Homozygous State

Normal

Phenotype Heterozygous State

None

Original Genetic Background

129S1/Sv-p+Tyr+KitlSl-J/+

Genetic Background Currently Maintained

129X1/SvJ

Coat Colour

white to light chinchilla

Eye Colour

red

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	Yes	Yes
Are homozygous mice fertile?	Yes	Yes
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Generations Backcrossed	3
Sib-Mated Generations	0
Mating Scheme	Backcrossed into 129X1/SvJ to increase health as ES origin is not as viable.
Homozygous Matings Required?	No
Development Summary	Blastocyst injection of ES cell (blastocysts were 129X1/SvJ), mosaic founders were crossed to 129X1/SvJ and offspring backcrossed to 129X1/SvJ for 3 generations
Percentage of Female Homozygous Mice Surviving to Maturity	100.0
Percentage of Male Hemizygous Mice Surviving to Maturity	100.0
Age of Reproductive Maturity	8 weeks
Age of Reproductive Decline	12 months
Male Fertility Compromised?	No
Average Number of Pups at Birth	6.0
Average Number of Pups Surviving to Weaning	6.0
Weaning Age	3 weeks
Number of Litters in Lifetime	Animal house policy to stop after 6 litters
Breeding Performance	Good
Number of Litters in Lifetime	Animal house policy to stop after 6 litters
Special Husbandry Requirements	General mouse husbandry, not special requirements

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Yes

Research value of this Strain

Strain models human congenital malformations and placental insufficiency.

Applicable Research Areas

Cardiovascular
Developmental biology
Models for human disease
Apoptosis

Keywords

Cited2
heart
placenta
left-right axis

Additional Information

Similar to C2f and C2Nf strains, however C2X1f does not contain neo compared to C2Nf strain and is on 129X1/SvJ background compared to C2f strain which is on mixed background

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 15 mice)

APB stock genotyped verified by:

The depositor

Are live mice available?

Unknown

MTA needs to be signed?

Yes