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Mouse Strain information

APB ID

2010

Added

17/09/2007

Last Edited

11/05/2014

Strain nomenclature:

B6;129P2-Stat3tm1Aki/Apb

Nickname:

Stat3 heterozygous knockout

Strain designation name:

B6;129P2-Stat3tm1Aki

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted knockout

ES Cell-Line Used

E14-1

Mode of Inheritance

Recessive

1. Affected Gene Name: Signal transducer and activator of transcription 3

Chromosome	11
Affected Gene Symbol	Stat3
Protein expression of altered gene:	Nil
Genetic alteration of gene:	Exons 20, 21, and 22 are replaced with the pMC1-neo gene (Stratagene). This deletion causes a loss of Src homology 2 domain and tyrosine residue, which are essential for activation of STAT proteins.
Location on chromosome (bp):	100746412-100800854 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:103038
Synonyms	Aprf
Allele Name	targeted mutation 1, Shizuo Akira
Allele Symbol	Stat3tm1Aki
MGI Allele Accession ID	MGI:1926814

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Embryonic lethal (E7.5)

Phenotype Heterozygous State

Viable, fertile - no apparent abnormalities.

Original Genetic Background

129Sv x C57BL/6

Genetic Background Currently Maintained

129Sv x C57BL/6

Coat Colour

Mixed agouti and black

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	No	No
Are homozygous mice fertile?	No	No
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Mating Scheme	heterozygous x heterozygous
Homozygous Matings Required?	No
Percentage of Female Homozygous Mice Surviving to Maturity	0.0
Percentage of Male Hemizygous Mice Surviving to Maturity	100.0
Age of Reproductive Maturity	7-8 weeks
Male Fertility Compromised?	No
Average Number of Pups at Birth	5.0
Average Number of Pups Surviving to Weaning	5.0
Weaning Age	3 weeks
Number of Litters in Lifetime	6
Breeding Performance	Good
Number of Litters in Lifetime	6
Special Husbandry Requirements	None

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Research value of this Strain

This is the only strain available to assess the impact of globally-reduced STAT3 activation in vivo.

Applicable Research Areas

Cell biology
Gastrointestinal
Haematology
Cancer
Cardiovascular
Bone
Apoptosis
Immunology and inflammation
Internal/organ
Research tools
Models for human disease

Keywords

STAT
cytokine
transcription
interleukin

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 9 mice)

APB stock genotyped verified by:

The depositor

Are live mice available?

Unknown

MTA needs to be signed?

Yes