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Mouse Strain information

APB ID

Added

06/02/2006

Last Edited

11/05/2014

Strain nomenclature:

C57BL/6JSfdAnu-Trp53Bhy/AnuApb

Nickname:

B-thy

Strain designation name:

C57BL/6JSfdAnu-Trp53Bhy/AnuApb

Genetics

Strain Types

Mutant strain
Coisogenic

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Semi-dominant

1. Affected Gene Name: transformation related protein 53

Chromosome

Affected Gene Symbol

Trp53

Protein expression of altered gene:

Nil

Genetic alteration of gene:

ENU induced mutation

Location on chromosome (bp):

69580359-69591873 bp, + strand From VEGA annotation of GRCm38

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:98834

Synonyms

p53

Allele Name

bthy

Allele Symbol

Trp53Bhy

MGI Allele Accession ID

MGI:3611889

Ensembl Gene ID:

ENSMUSG00000059552

Transcript ID:

ENSMUST00000108658

Transcript name:

Trp53-201

bp change:

T to C at position 540 on cDNA

Exon number:

Exon ID:

ENSMUSE00000111826

cDNA position:

540

Amino acid change:

Cysteine to Arginine at position 138

Sequence:
510	AATAAGCTATTCTGCCAGCTGGCGAAGACGTGCCCTGTGCAGTTGTGGGTCAGCGCCACAC	cDNA (forward strand)
128	-N--K--L--F--C--Q--L--A--K--T--C--P--V--Q--L--W--V--S--A--T--	protein

Version:

NCBI m37, Ensembl build 55

Mutant Construction Technique

None

Phenotype

Phenotype Heterozygous State

primitive hematopoietic or T cell leukemia, Sudden death, hemorrhage, Thymic lymphoma Fibrosarcoma Multinodal lymphoma Osteosarcoma Haemangiosarcoma Brain tumour Teratocarcinoma

Original Genetic Background

C57BL/6JSfdAnu

Genetic Background Currently Maintained

C57BL/6JSfdAnu

Coat Colour

Black

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

B-thy A –D11Mit349 (27.3cM)
B-thy B –D11Mit29 (37.2cM)

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

General information

Associated IP rights?

Does it Model a Human Condition?

Yes

Applicable Research Areas

Cancer

Keywords

p53
apoptosis
tumour
cell cycle
ENU

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 6 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

MTA needs to be signed?

Yes