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Mouse Strain information

APB ID

347

Added

25/09/2006

Last Edited

11/05/2014

Strain nomenclature:

B6;129S1-Cited2tm1.1Dunw/Apb

Nickname:

C2f

Strain designation name:

B6;129S1-Cited2tm1.1Dunw/Apb

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted reporter
Targeted floxed

ES Cell-Line Used

W9.5

Mode of Inheritance

Semi-dominant

1. Affected Gene Name: Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal

Chromosome	10
Affected Gene Symbol	Cited2
Protein expression of altered gene:	Nil
Genetic alteration of gene:	LoxP site inserted just upstream of the ATG, loxP-neo-loxP-lacZ cassette inserted downstream of exon 2. After recombinant neomycin resistant clones were selected, the neomycin resistance gene was deleted by partial Cre recombination in vitro. Cited2 expression replaced by lacZ reporter expression
Location on chromosome (bp):	17443032-17444750 bp, + strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:1306784
Synonyms	ER154-like, Mrg1, Msg2, p35srj
Allele Name	targeted mutation 1.1, Sally L Dunwoodie
Allele Symbol	Cited2tm1.1Dunw
MGI Allele Accession ID	MGI:3696062

Mutant Construction Technique

CRE recombinase-loxP technology

Phenotype

Phenotype Homozygous State

Mice homozygous for the conditional allele of Cited2 are viable and no phenotype has been observed.

Phenotype Heterozygous State

Normal

Original Genetic Background

ES cells (derived from mouse strain 129S1/Sv-p+Tyr+KitlSl-J/+ (Jax stock No. 000090)) were injected into C57BL/6 blastocysts

Genetic Background Currently Maintained

75% C57BL/6, 25% 129S1/Sv

Coat Colour

Black

Eye Colour

Black

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

To Distinguish the Cited2 wildtype allele from C2F Conditional Allele

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	Yes	Yes
Are homozygous mice fertile?	Yes	Yes
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Generations Backcrossed	2
Sib-Mated Generations	2
Mating Scheme	Homozygous sib-matings to propagate the line in the homozygous state
Homozygous Matings Required?	No
Development Summary	Blastocyst injection of ES cell (blastocysts were 129X1/SvJ), mosaic founders were crossed to C57BL/6 and offspring backcrossed to C57BL/6 for one more generation. Then sib-mated to derive homozygous offspring and homozygous offspring sib-mated to propagate the line in the homozygous state.
Age of Reproductive Maturity	8 weeks
Age of Reproductive Decline	unknown
Male Fertility Compromised?	No
Average Number of Pups at Birth	8.0
Average Number of Pups Surviving to Weaning	8.0
Weaning Age	3
Number of Litters in Lifetime	6
Breeding Performance	Good
Number of Litters in Lifetime	6

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Yes

Research value of this Strain

Models human congenital malformations and placental insufficiency. Once mated with cre recombinase expressing mice, heart and placental defects are expected like that of Cited2 null allele.

Applicable Research Areas

Apoptosis
Developmental biology
Models for human disease
Cardiovascular

Keywords

Cited2
heart
placenta
left-right axis

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 13 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

MTA needs to be signed?

Yes