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Mouse Strain information

APB ID

4511

Added

26/02/2009

Last Edited

11/05/2014

Strain nomenclature:

C57BL/10JAnu-Prdx1flro/AnuApb

Nickname:

fluoro ; ENU8B6:058

Genetics

Strain Types

Mutant strain

Genetic Details

Spontaneous

Mode of Inheritance

Recessive

1. Affected Gene Name: peroxiredoxin 1

Chromosome

Affected Gene Symbol

Prdx1

Protein expression of altered gene:

Unknown

Genetic alteration of gene:

Spontaneous mutation, missense

Location on chromosome (bp):

116358149-116373427 bp, + strand (From VEGA annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:99523

Synonyms

macrophage 23kDa stress protein, macrophase stress protein 22kDa, MSP23, OSF-3, osteoblast specific factor 3, PAG, Paga, Prx I, PrxI, Tdpx2, TDX2, thioredoxin dependent peroxide reductase 2, Trx dependent peroxide reductase 2

Allele Name

fluoro

Allele Symbol

Prdx1flro

MGI Allele Accession ID

MGI:4819263

Ensembl Gene ID:

ENSMUSG00000005161

Transcript ID:

ENSMUST00000005292

Transcript name:

Prdx2-001

bp change:

C to G at position 301 on cDNA

Exon number:

Exon ID:

ENSMUSE00000499125

cDNA position:

301

Amino acid change:

Serine to Cysteine at position 79

Sequence:
271	GCTGCGAGGTGCTGGGAGTGTCTGTGGACTCTCAGTTCACCCACCTGGCGTGGATCAATAC	cDNA (forward strand)
69	G--C--E--V--L--G--V--S--V--D--S--Q--F--T--H--L--A--W--I--N--T	protein

Version:

NCBI m37, Ensembl build 56

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Autofluorescence, multiple wavelengths, in flow cytometry of all lymphocyte subsets and erythrocytes.

Original Genetic Background

C57BL/10

Genetic Background Currently Maintained

C57BL/10

Strain Images

Flow cytometry plot of cells from affected animals. No antibodies added.

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

Fluoro

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Haematology

Keywords

lymphocyte
erythrocyte, red blood cell
ENU
Wellcome Trust

Additional Information

Discovered by NIH B cell proliferation screen and Wellcome Trust flow cytometry screens by Dr Lina Tze and Dr Katrina Randall (JCSMR, ANU). Mapped and mutation identified by Dr Katrina Randall and Belinda Whittle. Project guided by Prof Chris Goodnow.

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 11 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

Unknown

MTA needs to be signed?

Yes