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Mouse Strain information

APB ID

4721

Added

08/04/2009

Last Edited

14/05/2014

Strain nomenclature:

C57BL/6-Tgm2tm1Rmgr/Apb

Nickname:

TGM2 floxed

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted floxed

ES Cell-Line Used

W9.5/W95 isolated from 129/Sv strain

Mode of Inheritance

Recessive

1. Affected Gene Name: transglutaminase 2, C polypeptide

Chromosome	2
Affected Gene Symbol	Tgm2
Protein expression of altered gene:	Normal
Genetic alteration of gene:	LoxP sites that flank exons 6-8 were inserted by homologous recombination.
Location on chromosome (bp):	157942138-157972172 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:98731
Synonyms	G[a]h, protein-glutamine gamma-glutamyltransferase, TG C, TG2, tissue transglutaminase, tTG, tTGas
Allele Name	targeted mutation 1, Robert M Graham
Allele Symbol	Tgm2tm1Rmgr
MGI Allele Accession ID	MGI:3525256
Allele synonyms:	Tgm2t

Mutant Construction Technique

CRE recombinase-loxP technology

Phenotype

Phenotype Homozygous State

Normal - no phenotypic analysis * mice were only used to generate the null allele

Original Genetic Background

129S1/SvImJ x C57BL/6

Genetic Background Currently Maintained

C57BL/6

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

TGM2 flox

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Research value of this Strain

Many important biological processes, such as blood coagulation, are dependent on rapid cross-linking or "spotwelding". These reactions, which are catalysed by transglutaminases, endow the resulting structure with extra rigidity and resistance against proteolytic degradation. Having knowledge of these enzymes is essential for understanding diverse hereditary diseases of the blood and skin and various autoimmune, inflammatory and degenerative conditions. One such enzyme, tranglutaminase type 2 (TGM2) has been implicated in wound healing and in the pathogenesis of inflammatory disorders such as Crohn's disease, ulcerative colitis and coeliac disease as well as a number of important neurodegenerative diseases, such as Huntington's, Alzheimer's and progressive suprabulbar palsy. TG2 is unique among the transglutaminases because it is the only member shown to have functions other than cross-linking. The various functions of TG2 must be tightly coordinated. To better understand the function of TG2, we utilised homologous recombination to develop a floxed mouse line in which the TGM2 gene can be inactivated by Cre-mediated excision, either in the whole organism or in tissue-specific manner.

Applicable Research Areas

Immunology and inflammation
Cancer
Apoptosis
Neurobiology
Diabetes
Cardiovascular

Keywords

Transgluatminase
Gh
G-protein
cross-linking

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by:

The depositor

Are live mice available?

MTA needs to be signed?

Yes