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Mouse Strain information

APB ID

4756

Added

30/04/2009

Last Edited

14/05/2014

Strain nomenclature:

STOCK Tg(CAG-EGFP)1Osb/MarpApb

Nickname:

NOD eGFP

Genetics

Strain Types

Mutant strain

Genetic Details

Transgenic

Mode of Inheritance

Dominant

1. Transgene: transgene insertion 1, Research Institute for Microbial Diseases, Osaka University

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Transgenic mice were uniformly green with the exception of hair and red blood cells. The brain, liver, kidney, adrenal gland and testis, lung, muscle, heart, intestine, and adipose tissue, thymus, spleen and testicular cells of all transgenic mouse lines were naturally green when irradiated with excitation light.

Original Genetic Background

BCF1

Genetic Background Currently Maintained

NOD x C57BL/6

Coat Colour

White

Eye Colour

Red

Strain identification

How is this strain characterised?

Phenotyping

Characteristics Assayed For

Mice positive for GFP can be identified by exposing them to UV light. A positive mouse will fluoresce green which can be seen with the naked eye.

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	Yes	Yes
Are homozygous mice fertile?	Yes	Yes
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Homozygous Matings Required?	No
Development Summary	the strain was originally on a C57Bl/6 background when Frank Alderuccio and Ban-Hock Toh received them and they backcrossed them onto a Balb/cCrSlc background. The Aust. lab received the mice when they were at the 10th generation backcross to Balb/cCrSlc and commenced backcrossing the mice to the NOD background.
Male Fertility Compromised?	No
Average Number of Pups at Birth	10.0
Average Number of Pups Surviving to Weaning	10.0
Weaning Age	21 days
Breeding Performance	Good

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Models for human disease

Keywords

GFP
expression

Additional Information

Imported from Frank Alderuccio and Ban-Hock Toh, Monash University as Balb/c eGFP

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

MTA needs to be signed?