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Mouse Strain information

APB ID

4879

Added

28/09/2009

Last Edited

14/05/2014

Strain nomenclature:

C.B6-Rag1tm1Mom/ArcStvApb

Nickname:

Bc.Rag1.KO

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted knockout

Mode of Inheritance

Recessive

1. Affected Gene Name: recombination activating gene 1

Chromosome	2
Affected Gene Symbol	Rag1
Protein expression of altered gene:	Nil
Genetic alteration of gene:	A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.
Location on chromosome (bp):	101478439-101489658 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:97848
Synonyms	Rag-1
Allele Name	targeted mutation 1, Peter Mombaerts
Allele Symbol	Rag1tm1Mom
MGI Allele Accession ID	MGI:1857241

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Homozygotes for targeted null mutations exhibit arrested development of T and B cell maturation at the CD4-8- thymocyte or B220+/CD43+pro-B cell stage due to inability to undergo V(D)J recombination.

Original Genetic Background

129S7 x C57BL/6

Genetic Background Currently Maintained

BALB/c x C57BL/6

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	Yes	Yes
Are homozygous mice fertile?	Yes	Yes
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Generations Backcrossed	6
Homozygous Matings Required?	No
Development Summary	Strain obtained from ARC and further back-crossed to BALB/c
Male Fertility Compromised?	No
Breeding Performance	Unknown
Special Husbandry Requirements	This strain should be housed under pathogen free conditions

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation
Haematology
Cell biology

Keywords

T cell
B cell
eosinophil
inflammation
interferon

Additional Information

ARC source: CULAS, 1997. Placed directly behind barrier as SPF stock.

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

Unknown

MTA needs to be signed?

Yes