A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.
A 1.4-kb Sal fragment containing the neoR resistance gene flanked by yeast FLP recombinase target sites (16) was inserted into a unique SalI site within exon 9.
Homozygotes for targeted null mutations exhibit arrested development of T and B cell maturation at the CD4-8- thymocyte or B220+/CD43+pro-B cell stage due to inability to undergo V(D)J recombination.
All blood/tissue samples lack Gal expression.
Cortical cataracts developed in all GalT-/- mice at 4-6 weeks of age.
Original Genetic Background
129S7, CBA x C57BL/6
Genetic Background Currently Maintained
C57BL/6
Coat Colour
Black
Eye Colour
Black
Strain identification
How is this strain characterised?
Phenotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Female
Male
Are homozygous mice viable?
Yes
Yes
Are homozygous mice fertile?
Yes
Yes
Are heterozygotes / hemizygotes* fertile?
Yes
Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex
carrying an inserted transgene with no homologous allele on the other chromosome.
Homozygous Matings Required?
No
Development Summary
Cross of C57BL/6-Rag1tm1Mom and C57BL/6-Ggta1tm1 strains
Male Fertility Compromised?
No
Breeding Performance
Unknown
Special Husbandry Requirements
This strain should be housed under pathogen free conditions
