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Mouse Strain information

APB ID

4887

Added

28/09/2009

Last Edited

14/05/2014

Strain nomenclature:

129-Elanetm1(cre)Roes/Apb

Nickname:

129-Elane

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted knockin

Mode of Inheritance

Dominant

1. Affected Gene Name: elastase, neutrophil expressed

Chromosome	10
Affected Gene Symbol	Elane
Genetic alteration of gene:	A construct containing a cre recombinase gene, in which GC pairs had been deleted, and a floxed neo gene, was incoporated at the start codon of the endogenous gene. The floxed neo gene was excised in vitro by transiently transfecting ES cells with cre expression vectors. Neither transcript nor protein were detected by Northern and Western blot analyses. Expression of the modified cre transgene permits conditional mutagenesis at sites tagged with loxP in myeloid precursor cells.
Location on chromosome (bp):	79349057-79350961 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:2679229
Synonyms	Ela2, F430011M15Rik, NE, neutrophil elastase
Allele Name	targeted mutation 1, Jurgen Roes
Allele Symbol	Elanetm1(cre)Roes
MGI Allele Accession ID	MGI:2182177
Allele synonyms:	ElaCre, GE-, GEcre, NE-

1. Transgene: Cre recombinase

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Decreased susceptibility to bacterial infection: median survival time is increased to 477 +/- 88 min compared to 435 +/- 8 min in wild-type mice Increased susceptibility to fungal infection: after infection with 500,000 A. fumigatus spores only 60% of homozygotes survived more than 21 days compared to all wild-type mice surviving 6 days after infection kidney fungal load (304 mean CFU) is higher than in wild-type (16 mean CFU) but lower than in Ctsg and Ela double homozygotes (1009 mean CFU)

Original Genetic Background

129P2/OlaHsd + 129S2/SvPas

Genetic Background Currently Maintained

129X1/Sv

Coat Colour

Light Bellied Black Agouti

Eye Colour

Black

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

neutrophil
serine protease
reactive oxygen intermediates
endotoxin, LPS
TNF alpha
elastase

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 10 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

Unknown

MTA needs to be signed?