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Mouse Strain information

APB ID

5182

Added

22/07/2010

Last Edited

19/05/2014

Strain nomenclature:

B6.129-Ggntm1Dpj/42MarpApb

Nickname:

Ggn Knockout clone 42

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted knockout

ES Cell-Line Used

129Sv

Mode of Inheritance

Recessive

1. Affected Gene Name: gametogenetin

Chromosome	7
Affected Gene Symbol	Ggn
Genetic alteration of gene:	The gene is an intron-less gene so a Neo cassette was inserted to and replaced 98% of the coding region. Western blot analysis confirmed reduced protein expression in spermatocytes from heterozygotes. Two clones were characterised #16 and 42.
Location on chromosome (bp):	29170220-29173933 bp, + strand From Ensembl annotation of GRCm38
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:2181461
Synonyms	GGN1, GGN2, GGN3
Allele Name	gametogenetin; targeted mutation 1, Duangporn Jamsai
Allele Symbol	Ggntm1Dpj
MGI Allele Accession ID	MGI:5503309

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Embryonic lethal

Phenotype Heterozygous State

Appear normal. Abnormal double-strand DNA break repair: • increased unrepaired double-strand breaks in pachytene spermatocytes

Original Genetic Background

129Sv

Genetic Background Currently Maintained

C57BL/6J x 129Sv

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Reproduction

Keywords

sperm
testis
embryonic lethal

Is the strain available in any form to other researchers

Possibly

APB stock stored as:

Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

Unknown

MTA needs to be signed?

Yes