Home - Australian Phenome Bank

Mouse Strain information

APB ID

5217

Added

14/09/2010

Last Edited

19/05/2014

Strain nomenclature:

129/SvJ-Xrcc6tm1Fwa/AnuApb

Nickname:

Ku70 null 129/SvJ

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted knockout

ES Cell-Line Used

TC-1

Mode of Inheritance

Recessive

1. Affected Gene Name: X-ray repair complementing defective repair in Chinese hamster cells 6

Chromosome	15
Affected Gene Symbol	Xrcc6
Protein expression of altered gene:	Nil
Genetic alteration of gene:	450 bp of this gene encompassing exon 4 and part of exon 5 were replaced with a neo cassette via homologous recombination resulting in a shift in the reading frame and predicted protein truncation after the first 64 residues. Homozygous mutant ES cells were identified by Southern blot analysis and Western blot analysis confirmed the absence of protein product.
Location on chromosome (bp):	81846825-81870514 bp, + strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:95606
Synonyms	G22p1, Ku p70, Ku70
Allele Name	X-ray repair complementing defective repair in Chinese hamster cells 6; targeted mutation 1, Frederick W Alt
Allele Symbol	Xrcc6tm1Fwa
MGI Allele Accession ID	MGI:2179954
Allele synonyms:	Ku70-, Ku70DKO

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Smaller than littermate controls (about 50% reduction in size). Ku70 null male and females are infertile; they also have impaired T cell development with 1% developing thymic or T cell lymphomas by 9 mths.

Phenotype Heterozygous State

Normal

Original Genetic Background

129/SvJ

Genetic Background Currently Maintained

129/SvJ

Coat Colour

Brown

Eye Colour

Brown

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	Yes	Yes
Are homozygous mice fertile?	No	No
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Mating Scheme	Ku70 het to Ku70 het crossings required
Homozygous Matings Required?	No
Male Fertility Compromised?	Yes
Average Number of Pups at Birth	5.0
Average Number of Pups Surviving to Weaning	5.0
Breeding Performance	Poor
Special Husbandry Requirements	SPF facility, autoclave food and water. Susceptible to infections due to T cell defect. Rarely, diarrhoea (animal otherwise active and well) or penile prolapse (if this occurs, humanely euthanise).

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Other Patent Technologies?

Associated IP rights?

Unknown

Does it Model a Human Condition?

Yes

Research value of this Strain

Model for immunodeficiency syndromes and accelerated hepatocellular carcinoma.

Applicable Research Areas

Cancer
Developmental biology
Gastrointestinal
Apoptosis
Models for human disease

Keywords

T cell
hepatocyte
hepatocellular carcinoma (HCC)
DNA-dependent protein kinase (DNA-PK)
repair
T cell
immunoglobulin
V(D)J
lymphocyte

Is the strain available in any form to other researchers

Possibly

APB stock stored as:

Cryopreserved sperm (from 14 mice)

Are live mice available?

Unknown

MTA needs to be signed?

Yes