Mouse Strain information
APB ID 5286
Added 28/10/2010
Last Edited 28/02/2017
Strain nomenclature: C57BL/6-Nt5etm1Lft
Nickname: B6.CD73.KO
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: 5' nucleotidase, ecto  
Chromosome 9
Affected Gene Symbol Nt5e
Protein expression of altered gene: Nil
Genetic alteration of gene: Exon 3 was replaced with a neo cassette in reverse orientation to transcription. Northern blot of kidneys from mutant animlas indicated a lack of transcript.
Location on chromosome (bp): 88222478-88266927 bp, + strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

 MGI:99782
Synonyms CD73, ecto-5'-nucleotidase, Nt5
Allele Name 5' nucleotidase, ecto; targeted mutation 1, Linda F Thompson
Allele Symbol Nt5etm1Lft
MGI Allele Accession ID MGI:3522017
Allele synonyms: Cd73-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Increased aggression toward humans: under hypoxic conditions mutants are less compliant compared to wild-type littermates. Lethargy: under hypoxic conditions mutants are more lethargic compared to wild-type littermates. Increased vascular permeability: * under hypoxic conditions increased vascular leakage is seen. * under normoxic conditions increased vascular permeability is seen in the lung and colon. Altered response to myocardial infarction: * ischemic preconditioning fails to attenuate infarct size as in wild-type mice. * however, treatment of mice with adenosine or 5' nucleotidase infusion partially-restores cardioprotection offered by ischemic preconditioning. Increased infarction size: mice exhibit increased infarct size after 60 minutes of ischemia compared to in similarly-treated wild-type mice. Edema: under hypoxic conditions increased edema is seen in all organs except the brain. Abnormal response/metabolism to endogenous compounds: mice exhibit an attenuated bradycardia response to AMP.
Original Genetic Background 129S1/Sv
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? Unknown
Does it Model a Human Condition? Yes
Applicable Research Areas Internal/organ
Keywords
  • ischemia
  • infarction
  • myocardium
  • hypoxia
Additional Information Permission from owner required.
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 5 mice)
Are live mice available? Unknown
MTA needs to be signed? Yes


     

 


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