Mouse Strain information
APB ID 5320
Added 16/11/2010
Last Edited 13/03/2025
Strain nomenclature: B6,129-Robo1tm1.1Ljr/Apb
Nickname: Robo 1 KO
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
ES Cell-Line Used 129/Sv-derived
Mode of Inheritance Dominant
1.  Affected Gene Name: roundabout homolog 1 (Drosophila)  
Chromosome 16
Affected Gene Symbol Robo1
Protein expression of altered gene: Nil
Genetic alteration of gene: Exon 5 was removed via cre-mediated recombination. RT-PCR confirmed absence of wild-type transcript in mutants. Western blot failed to detect protein.
Location on chromosome (bp): 72663394-73046340 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

 MGI:1274781
Synonyms DUTT1
Allele Name roundabout homolog 1 (Drosophila); targeted mutation 1.1, Linda J Richards
Allele Symbol Robo1tm1.1Ljr
MGI Allele Accession ID MGI:3641236
Allele synonyms: Robo1-
Point mutation sequence:
Insertion/Tagging details:
Guide(s) RNA sequence:
Mutant Construction Technique CRE recombinase-loxP technology
Phenotype
Phenotype Homozygous State Malformation in the corpus callosum (axons tracing a dorso-ventral path instead of medio-lateral). Disruption of the hippocampal commissure. Fornix malformation. Some disruption of midline zipper glia.
Phenotype Heterozygous State Normal callosal projections.
Original Genetic Background 129 ES Cells, subsequently bred on C57BL6
Genetic Background Currently Maintained C57BL/6
Coat Colour Black
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? Unknown
Does it Model a Human Condition? Yes
Description of human condition: Agenesis of the corpus callosum.
Research value of this Strain Demonstrates the efffect of removing a guidance receptor on midline commissure development. Demonstrates individual and separate roles of individuals within a receptor family. Also useful in examining interactions between the receptor DCC and Robo1.
Applicable Research Areas Neurobiology
Models for human disease
Keywords
  • axon
  • interneuron migration
  • corticothalamic
  • thalamocortical
  • Slit
Is the strain available in any form to other researchers Possibly
APB stock stored as: Cryopreserved sperm (from 5 mice)
Are live mice available? No
MTA needs to be signed? Yes


     

 


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