Mouse Strain information
APB ID
5334
Added
01/12/2010
Last Edited
06/06/2014
Strain nomenclature:
NOD-B2mtm1Jae Tg(B2m)1Rms Tg(TcraTcrbNY8.3)1Pesa/MarpApb
Nickname:
NODβ2M-/- flβ2Ma 8.3TCRαβ
Genetics
Strain Types
Mutant strain
Genetic Details
Transgenic
Mode of Inheritance
Recessive
1. Affected Gene Name:
beta-2 microglobulin
Chromosome
2
Affected Gene Symbol
B2m
Protein expression of altered gene:
Unknown
Genetic alteration of gene:
A neomycin resistance cassette was inserted into the second exon.
Location on chromosome (bp):
121973422-121978819 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
MGI:88127
Synonyms
beta 2 microglobulin, beta2-m, Ly-m11
Allele Name
targeted mutation 1, Rudolf Jaenisch
Allele Symbol
B2mtm1Jae
MGI Allele Accession ID
MGI:1927183
Allele synonyms:
B2M-, beta2M-, beta2mnull , MHC classI-
2. Affected Gene Name:
Unknown
Chromosome
Unknown
Genetic alteration of gene:
B2M a transgene was microinjected directly into NOD/Lt zygotes.
1. Transgene:
transgene insertion 1, Robyn M Slattery
Symbol
Tg(B2ma)1Rms
Promoter
B2m
Transgene Synonyms:
beta2Ma
Genetic alteration of transgene:
The B2Mb gene derived from the plasmid pKC7-B2Mb was converted to the B2Ma form of the gene by site directed mutagenesis.
A B2m genomic sequence containing loxP sites flanking exons 2 and 3 was used for the transgene.
MGI Accession ID:
MGI:2663951
2. Transgene:
transgene insertion 1, Pere Santamaria
Symbol
Tg(TcraTcrbNY8.3)1Pesa
Promoter
TCR
Transgene Synonyms:
8.3-TCR, 8.3-TCR-alpha/beta transgene, 8.3-TCR-TG, 8.3TCR, NY8.3, Tcr8.3
Genetic alteration of transgene:
Functional VDJbeta and VJalpha rearrangements were isolated from the beta cell cytotoxic CD8(+) T cell clone NY8.3. Upstream regualtory sequence together with either TCR-beta enhancers or TCR-alpha enhancers, as appropriate, were associated with VDJbeta or VJalpha.
MGI Accession ID:
MGI:3584121
Mutant Construction Technique
CRE recombinase-loxP technology
Phenotype
Phenotype Homozygous State
Unknown
Phenotype Heterozygous State
TCR transgenic mouse with accelerated diabetes.
Original Genetic Background
Mixed
Genetic Background Currently Maintained
NOD
Strain identification
How is this strain characterised?
Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Female
Male
Are homozygous mice viable?
Unknown
Unknown
Are homozygous mice fertile?
Unknown
Unknown
Are heterozygotes / hemizygotes* fertile?
Yes
Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex
Homozygous Matings Required?
No
Male Fertility Compromised?
No
Breeding Performance
Good
Relevant bibliographic / database references
Strain Specific References
1.
Dudek NL, Thomas HE, Mariana L, Sutherland RM, Allison J, Estella E, Angstetra E, Trapani JA, Santamaria P, Lew AM, Kay TW. Cytotoxic T-cells from T-cell receptor transgenic NOD8.3 mice destroy beta-cells via the perforin and Fas pathways. Diabetes. 2006 Sep;55(9):2412-8
2.
Verdaguer J, Schmidt D, Amrani A, Anderson B, Averill N, Santamaria P: Spontaneous autoimmune diabetes in monoclonal T-cell nonobese diabetic mice. J Exp Med 186:1663–1676, 1997
3.
Hamilton-Williams EE, Serreze DV, Charlton B, Johnson EA, Marron MP, Mullbacher A, Slattery RM. Transgenic rescue implicates beta2-microglobulin as a diabetes susceptibility gene in nonobese diabetic (NOD) mice. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11533-8. Erratum in: Proc Natl Acad Sci U S A 2001 Nov 6;98(23):13472.
Other Related References
General information
Associated IP rights?
No
Does it Model a Human Condition?
Unknown
Applicable Research Areas
Immunology and inflammation
Keywords
Autoimmunity
insulin
MHC I
beta cell
Is the strain available in any form to other researchers
Possibly
APB stock stored as:
Cryopreserved sperm (from 5 mice)
APB stock genotyped verified by:
The depositor
Are live mice available?
Limited
MTA needs to be signed?
Yes
