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Mouse Strain information

APB ID

5381

Added

06/01/2011

Last Edited

13/03/2025

Strain nomenclature:

C57BL/6-Tg(CAG-EGFP)131Osb/LudApb

Nickname:

MF-04 ; EGFP

Genetics

Strain Types

Mutant strain

Genetic Details

Transgenic

Mode of Inheritance

Dominant

1. Transgene: transgene insertion 131, Research Institute for Microbial Diseases, Osaka University

Symbol	Tg(CAG-EGFP)131Osb
Promoter	ytomegalovirus immediate early + chicken beta-actin gene + rabbit hemoglobin beta gene
Transgene Expression Level	Ubiquitous
Transgene Synonyms:	Osb-Y01, Tg(Act-EGFP)C14-Y01-FM131Osb, Tg(act-EGFP)Y01Osb, Tg(ACTB-EGFP)131Osb, Tg(ACTB-EGFP)Y01Osb, Tg(CAG-EGFP)C14-Y01-FM131Osb, Tg14(act-EGFP)ObsY01, TgN(act-EGFP)ObsC14-Yo1-FM1310, Y01
Genetic alteration of transgene:	The transgene comprises the cDNA encoding enhanced green fluorescent protein (EGFP), from the jellyfish Aequoria victoria, downstream of the "CAG promoter" -- which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene -- and followed by the rabbit beta-hemoglobin polyadenylation signal and 3' flanking sequence. This transgene was made using the same construct as Tg(ACTB-EGFP)1Osb, but in this case it was injected into C57BL/6 fertilized oocytes. According to the Center for Animal Resources and Development Database (CARD R-Base), which distributes it, the transgene integrated on Chr 14D1.
MGI Accession ID:	MGI:3055151

Point mutation sequence:

Insertion/Tagging details:

Guide(s) RNA sequence:

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Ubiquitous expression of EGFP.

Phenotype Heterozygous State

Ubiquitous expression of EGFP but weaker signal than homozygote.

Original Genetic Background

C57BL/6

Genetic Background Currently Maintained

C57BL/6

Strain identification

How is this strain characterised?

Phenotyping
Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Yes

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Developmental biology
Research tools

Keywords

Additional Information

Following website provides more information about all of the clones generated. http://133.1.15.131/tg/greenmouse.cfm?link='Home%20of%20Original%20Green%20Mouse

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 5 mice)

Are live mice available?

MTA needs to be signed?

Yes