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APB

Australian Phenome Bank Database

The Australian Phenome Bank is part of Phenomics Australia

"A repository of genetically modified mouse strains held in Australia

either as live or cryopreserved stock"

Mouse Strain information

APB ID

5398

Added

27/01/2011

Last Edited

06/06/2014

Strain nomenclature:

B6;CBA-Tg(KRT1-Shh)/Apb

Nickname:

HK1-Shh

Genetics

Genetic Details

Transgenic

Mode of Inheritance

Dominant

1. Transgene: rat sonic hedgehog

Symbol	HK1-Shh
Promoter	human keratin 1
Transgene Expression Level	increased
Transgene Synonyms:	Krt1-Shh
Genetic alteration of transgene:	ClaI linkered primers (Shhfmk2 5′-ATC GAT ACC ACC ATG CTG CTG CTG CTG GC-3′, Shh1r 5′-ATC GAT TAG CTG GAC TGG ACT GCC-3′) were used to amplify the rat Shh complete open reading frame from nucleotides 315-1626 (GenBank Accession no. L27340). This product was subcloned into pGEM-T and sequenced completely with M13 forward and reverse primers and primers within Shh (Shh#3 5′-GGC CGA TAT GAA GGG AAG AT-3′, Shh#4 5′-ATC AGC CAC AGT GCA CCT G-3′, Shh#5 5′-TGT ACG TGG TGG CTG AAC G-3′, Shh#6 5′-TCT CGT AGC GTT ACG CTG TG-3′, Shh#7 5′-AGG AAG GTG AGG AAG TCG CTG-3′). This product was then subcloned into the ClaI site of the HK1 transgene (Rosenthal et al., 1991). The HK1 transgene construct is based on a 12-kb EcoRI fragment of the human Keratin 1 gene as previously described (Greenhalgh et al., 1993a,b). Transgenic mice were generated by pronuclear injection of embryos from a CBA/C57B16 F1 cross as described (Hogan et al., 1994). Transgenic mice were bred onto FVB or C57B16 backgrounds. All mice in this study were heterozygous for the transgene. DNA was screened for the Shh transgene by using primers which span intron 1 (Shh1F 5′-GCG ATT TAA GGA ACT CAC CCC C-3′, Shh2R 5′-TGC TTT CAC CGA GCA GTG G-3′).

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Unknown

Phenotype Heterozygous State

skin anomalies, fore- and hindlimp abnormalities

Original Genetic Background

CBA X C57BL/6

Genetic Background Currently Maintained

C57BL/6

Coat Colour

Black

Strain identification

How is this strain characterised?

Genotyping
Phenotyping

Suggested existing PCR names:

HK1

Fertility and Strain maintenance

Fertility and Strain maintenance

	Female	Male
Are homozygous mice viable?	Unknown	Unknown
Are homozygous mice fertile?	Unknown	Unknown
*Are heterozygotes / hemizygotes fertile?**	Yes	Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Mating Scheme	het x wt
Homozygous Matings Required?	No
Male Fertility Compromised?	No
Average Number of Pups at Birth	8.0
Average Number of Pups Surviving to Weaning	8.0
Breeding Performance	Good

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

No

Research value of this Strain

Model for overexpression of Hedgehog signalling

Applicable Research Areas

Developmental biology

Keywords

Sonic Hedgehog
skin
limb
embryonic development
overexpression

Is the strain available in any form to other researchers

Possibly

APB stock stored as:

Cryopreserved sperm (from 6 mice)

Are live mice available?

No

MTA needs to be signed?

No

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