Mouse Strain information
APB ID
5408
Added
03/02/2011
Last Edited
14/03/2025
Strain nomenclature:
STOCK Il6sttm1Ern Stat3tm1Aki Stat1tm1Rds /LudApb
Nickname:
Gp130Y757f / Stat3+/- / Stat1-/-
Genetics
Strain Types
Mutant strain
Genetic Details
Targeted knockout
Mode of Inheritance
Recessive
1. Affected Gene Name:
interleukin 6 signal transducer
Chromosome
13
Affected Gene Symbol
Il6st
Protein expression of altered gene:
Unknown
Genetic alteration of gene:
The Ptpn11 binding domain, encoded by sequence in exon 17, was disrupted by homologous recombination of a construct containing missense mutations at codons 757 (tyrosine to phenylalanine) and 760 (valine to alanine). Furthermore, an IRES-neo cassette was inserted into the 3' region of exon 17. A biochemical assay of liver tissue showed that the Il6st mediated signalling pathway was disrupted in mutant mice.
Location on chromosome (bp):
113265216-113297068 bp, + strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
MGI:96560
Synonyms
CD130, D13Ertd699e, gp130
Allele Name
interleukin 6 signal transducer; targeted mutation 1, Matthias Ernst
Allele Symbol
Il6sttm1Ern
MGI Allele Accession ID
MGI:2388478
Allele synonyms:
gp130757F , gp130Y757F , gp130Y757F
2. Affected Gene Name:
signal transducer and activator of transcription 3
Chromosome
11
Affected Gene Symbol
Stat3
Protein expression of altered gene:
Unknown
Genetic alteration of gene:
A neomycin resistance cassette replaced 3.0kb of sequence containing exons 20-22.
Location on chromosome (bp):
100746412-100800854 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
MGI:103038
Synonyms
Aprf
Allele Name
signal transducer and activator of transcription 3; targeted mutation 1, Shizuo Akira
Allele Symbol
Stat3tm1Aki
MGI Allele Accession ID
MGI:1926814
Allele synonyms:
Stat3-
3. Affected Gene Name:
signal transducer and activator of transcription 1
Chromosome
1
Affected Gene Symbol
Stat1
Genetic alteration of gene:
A neomycin resistance cassette replaced the first three coding exons and 0.7kb of 5' upstream flanking sequence.
Location on chromosome (bp):
52176282-52218704 bp, + strand (From Ensembl annotation of NCBI Build 37)
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
MGI:103063
Allele Name
signal transducer and activator of transcription 1; targeted mutation 1, Robert D Schreiber
Allele Symbol
Stat1tm1Rds
MGI Allele Accession ID
MGI:1861949
Allele synonyms:
S1KO, STAT1-
Point mutation sequence:
Insertion/Tagging details:
Guide(s) RNA sequence:
Mutant Construction Technique
None
Phenotype
Phenotype Homozygous State
Unknown
Phenotype Heterozygous State
Unknown
Original Genetic Background
129S1/Sv ; 129P2/OlaHsd ; 129/Sv
Genetic Background Currently Maintained
C57BL/6 x 129/Sv
Strain identification
How is this strain characterised?
Genotyping
PCR protocols
Stat3
Gp130dStat
Stat1
Fertility and Strain maintenance
Fertility and Strain maintenance
Female
Male
Are homozygous mice viable?
Yes
Yes
Are homozygous mice fertile?
Yes
Yes
Are heterozygotes / hemizygotes* fertile?
Yes
Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex
Homozygous Matings Required?
No
Development Summary
Stat1 x gp130Y757F/Y757F; Stat3. Progeny was then mated together.
Male Fertility Compromised?
No
Breeding Performance
Unknown
Relevant bibliographic / database references
Strain Specific References
1.
Tebbutt NC; Giraud AS; Inglese M; Jenkins B; Waring P; Clay FJ; Malki S; Alderman BM; Grail D; Hollande F; Heath JK; Ernst M, Reciprocal regulation of gastrointestinal homeostasis by SHP2 and STAT-mediated trefoil gene activation in gp130 mutant mice., Nat Med 2002 Oct;8(10):1089-97
2.
Takeda K; Noguchi K; Shi W; Tanaka T; Matsumoto M; Yoshida N ; Kishimoto T ; Akira S, Targeted disruption of the mouse Stat3 gene leads to early embryonic lethality., Proc Natl Acad Sci U S A 1997 Apr 15;94(8):3801-4
3.
Meraz MA; White JM; Sheehan KC; Bach EA; Rodig SJ; Dighe AS; Kaplan DH; Riley JK; Greenlund AC; Campbell D; Carver-Moore K; DuBois RN; Clark R; Aguet M; Schreiber RD, Targeted disruption of the Stat1 gene in mice reveals unexpected physiologic specificity in the JAK-STAT signaling pathway., Cell 1996 Feb 9;84(3):431-42
4.
Fielding CA; McLoughlin RM; McLeod L; Colmont CS; Najdovska M; Grail D; Ernst M; Jones SA; Topley N; Jenkins BJ, IL-6 regulates neutrophil trafficking during acute inflammation via STAT3., J Immunol 2008 Aug 1;181(3):2189-95
5.
Jenkins BJ; Grail D; Nheu T; Najdovska M; Wang B; Waring P; Inglese M; McLoughlin RM; Jones SA; Topley N; Baumann H; Judd LM; Giraud AS; Boussioutas A; Zhu HJ; Ernst M, Hyperactivation of Stat3 in gp130 mutant mice promotes gastric hyperproliferation and desensitizes TGF-beta signaling., Nat Med 2005 Aug;11(8):845-52
Other Related References
General information
Associated IP rights?
Yes
Does it Model a Human Condition?
Unknown
Applicable Research Areas
Immunology and inflammation
Keywords
signal transduction
kinase
acute peritoneal inflammation
neutrophil
Is the strain available in any form to other researchers
Yes
APB stock stored as:
Cryopreserved sperm (from 5 mice)
Are live mice available?
No
MTA needs to be signed?
Yes
