Home - Australian Phenome Bank

Mouse Strain information

APB ID

5416

Added

04/02/2011

Last Edited

24/03/2025

Strain nomenclature:

STOCK Apctm1Tno/Apb

Nickname:

APC (580s) ; MF-01

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted floxed

Mode of Inheritance

Dominant

1. Affected Gene Name: adenomatosis polyposis coli

Chromosome	18
Affected Gene Symbol	Apc
Protein expression of altered gene:	Reduced
Genetic alteration of gene:	One loxP site was inserted into intron 13 and the other, with a PGK-neo cassette, into intron 14. Northern blot analysis revealed that RNA expression in the intestinal tract of homozygous mutants was about 30% of that in wild-type mice. Protein expression in the colon of homozygotes by immunohistochemistry was indistinguishable from that of wild-type mice. Western blot analysis of lysates from mouse embryo fibroblasts showed reduced expression of the protein.
Location on chromosome (bp):	34380578-34478262 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:88039
Synonyms	CC1, Min
Allele Name	adenomatosis polyposis coli; targeted mutation 1, Tetsuo Noda
Allele Symbol	Apctm1Tno
MGI Allele Accession ID	MGI:1857966
Allele synonyms:	580S, Apc580S flox, Apc580s, Apcf, Apcfl, ApcloxP

Point mutation sequence:

Insertion/Tagging details:

Guide(s) RNA sequence:

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Normal until: Hepatocellular carcinoma: * all mutant mice (n=15) develop hepatocellular carcinomas by 450 days of age * hepatic tumor volumes in homozygous mice are larger than in heterozygous mice * no intestinal polyps (18 month, n=24)

Phenotype Heterozygous State

Normal

Original Genetic Background

129S4/SvJae

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

APC580s

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Yes

Does it Model a Human Condition?

Yes

Description of human condition:

Familial adenomatous polyposis coli (FAP)

Applicable Research Areas

Cancer

Keywords

Familial adenomatous polyposis coli (FAP)
colorectal adenomas
epithelium

Additional Information

Changed questions"Under the terms and conditions that you obtained this strain, do you have permission to submit the strain to the Australian Phenomebank for cryopreservation and archiving?" from NO to YES 24.03.25

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 5 mice)

Are live mice available?

MTA needs to be signed?

Yes