Mouse Strain information
APB ID 5454
Added 03/03/2011
Last Edited 06/06/2014
Strain nomenclature: C57BL/6-Phc2tm1Hko/WehiApb
Nickname: Phc2
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: polyhomeotic-like 2 (Drosophila)  
Chromosome 4
Affected Gene Symbol Phc2
Protein expression of altered gene: Nil
Genetic alteration of gene: A targeting vector was designed to replace exons 2 and 3 with a neomycin resistance cassette, resulting in the loss of the HDI region from the 36-kDa isoform. Northern blot showed the presence of three aberrant transcripts in mutant. However, immunoblot did not detect protein.
Location on chromosome (bp): 28331982-128430125 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

 MGI:1860454
Synonyms Edr2, Mph2
Allele Name polyhomeotic-like 2 (Drosophila); targeted mutation 1, Haruhiko Koseki
Allele Symbol Phc2tm1Hko
MGI Allele Accession ID MGI:3586989
Allele synonyms: Phc2-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Minor skeletal abnormalities. Female infertility Abnormal axial skeleton morphology: * all homozygotes have at least a few alterations to the axial skeleton characteristic of posterior transformations; however, the skull is similar to wild-type. * an ectopic bone floats between the occipital bones and the first cervical vertebra. Abnormal rib morphology. Increased rib number: ectopic ribs are associated with C7 in 67% of homozygotes. Rib fusion: C7 ectopic ribs are imperfect and fused to the middle part of the first ribs; the rest of the ribs are perfect and directly associated with the cranially shifted sternum. Abnormal sternum morphology: the sternum is shifted cranially. Asymmetric rib-sternum attachment: the 7th rib pair are detached from the sternum and the 13th rib pair are missing or floating. Fusion of atlas and odontoid process: the odontoid process is fused to the first cervical vertebra (C1). Split cervical atlas: the dorsal part of the C1 is often bifurcated. Split cervical axis: the dorsal part of C2 is often bifurcated. Vertebral transformation: the thoracolumbar and lumbosacral boundaries are shifted anteriorly. Thoracic vertebral transformation: a prominent spinous process normally associated with the second thoracic vertebra (T2) is associated with T1 in all homozygotes. Cervical vertebral transformation: in the lateral view C2 resembles C3. Decreased cell proliferation: MEFs grow more slowly and stop dividing earlier.
Phenotype Heterozygous State Normal
Original Genetic Background (129X1/SvJ x 129S1/Sv)F1
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? Unknown
Does it Model a Human Condition? Unknown
Applicable Research Areas Developmental biology
Bone
Keywords
  • Polycomb group proteins
  • polycomb repressive complex 1
  • Hox
  • skeleton
  • Cdkn2a
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 6 mice)
Are live mice available? Unknown
MTA needs to be signed? Yes


     

 


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