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Mouse Strain information

APB ID

6650

Added

22/11/2011

Last Edited

15/12/2022

Strain nomenclature:

C57BL/6N-PYYtm1(KOMP)Wtsi/Marp

Nickname:

APN 119

Genetics

Genetic Details

Targeted knockout
Targeted FRT

ES Cell-Line Used

ES cell clone ID: EPD0321_3__G04

Mode of Inheritance

Recessive

1. Affected Gene Name: peptide YY

Chromosome	11
Affected Gene Symbol	Pyy
Genetic alteration of gene:	The insertion of the L1L2_Bact_P cassette created a deletion of size 430 starting at position 101968278 and ending at position 101968708 of Chromosome 11 (Genome Build 37). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. (J:148605, J:173534)
Location on chromosome (bp):	Chr11:101967990-101969146 bp, - strand
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:99924
Synonyms	MGC:19143
Allele Name	peptide YY; targeted mutation 1, Wellcome Trust Sanger Institute
Allele Symbol	Pyytm1(KOMP)Wtsi
MGI Allele Accession ID	MGI:4363090

Point mutation sequence:

Insertion/Tagging details:

Guide(s) RNA sequence:

Mutant Construction Technique

CRE recombinase-loxP technology

Phenotype

Phenotype Homozygous State

Unknown - Not bred to homozygocity

Phenotype Heterozygous State

Normal

Original Genetic Background

C57BL/6N - JM8A1.N3

Genetic Background Currently Maintained

C57Bl/6N

Coat Colour

black/agouti

Eye Colour

Brown

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Additional Information

ES cell line imported from the Knockout Mouse Project Repository (KOMP, UC Davis, USA).

Is the strain available in any form to other researchers

Possibly

APB stock stored as:

Cryopreserved sperm (from 5 mice)

Are live mice available?

MTA needs to be signed?

Yes