Home - Australian Phenome Bank

Mouse Strain information

APB ID

6877

Added

24/04/2012

Last Edited

02/05/2023

Strain nomenclature:

C57BL/6-Abca1fl/flAbcg1fl/fl/Marp

Nickname:

Abca1fl/fl Abcg1fl/fl

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted floxed

Mode of Inheritance

Semi-dominant

1. Affected Gene Name: ATP-binding cassette, sub-family A (ABC1), member 1

Chromosome	4
Affected Gene Symbol	Abca1
Protein expression of altered gene:	Unknown
Genetic alteration of gene:	Conditional targeting of the mouse Abca1 gene was achieved by flanking exons 45–46, which encode the second nucleotide-binding fold, with loxP sites,
Location on chromosome (bp):	53043659-53172767 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:99607
Synonyms	Abca1

2. Affected Gene Name: ATP-binding cassette, sub-family G (WHITE), member 1

Chromosome	17
Affected Gene Symbol	Abcg1
Genetic alteration of gene:	Three loxP sites and one neomycin resistance gene were placed around exon 3, the exon that is deleted in general Abcg1-/- mice obtained from DeltaGen.4 The ABCG1 BAC construct was purchased from CHORI. The Frt_LoxP (FL) cassette (146 bp) was inserted at position 41200 and the Lox_Neo_Lox (LNL) cassette at position 41950. A 2 kb short arm downstream of the LNL cassette (ended at position 43950 before exon 4) was retrieved into a plasmid with the DTA cassette as the negative selection marker. A NotI site was engineered in front of the 5 kb long arm for the linearization of the targeting vector.
Location on chromosome (bp):	31194643-31252722 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:107704
Synonyms	Abc8, White

Point mutation sequence:

Insertion/Tagging details:

Guide(s) RNA sequence:

Mutant Construction Technique

CRE recombinase-loxP technology

Phenotype

Phenotype Homozygous State

Normal

Phenotype Heterozygous State

Normal

Original Genetic Background

C57BL/6J

Genetic Background Currently Maintained

C57BL/6J

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Does it Model a Human Condition?

Unknown

Is the strain available in any form to other researchers

Possibly

APB stock stored as:

Cryopreserved sperm (from 5 mice)

Are live mice available?

Unknown

MTA needs to be signed?