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Mouse Strain information

APB ID

6881

Added

02/05/2012

Last Edited

02/09/2015

Strain nomenclature:

C57BL/6JAnu-Mtorchino/AnuApb

Nickname:

ENU15Ch:020a:mTOR

Strain designation name:

C57BL/6JAnu-Mtorchino/AnuApb

Genetics

Strain Types

Mutant strain

Genetic Details

Chemically induced : ENU

Mode of Inheritance

Recessive

1. Affected Gene Name: mechanistic target of rapamycin (serine/threonine kinase)

Chromosome

Affected Gene Symbol

Mtor

Protein expression of altered gene:

Unknown

Genetic alteration of gene:

ENU-induced point mutation

Location on chromosome (bp):

147822720-147931792 bp, + strand (From VEGA annotation of NCBI Build 37)

MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

MGI:1928394

Synonyms

2610315D21Rik, FKBP-rapamycin-associated protein FRAP, flat, Frap1, RAFT1, RAPT1

Allele Name

mechanistic target of rapamycin (serine/threonine kinase); mutation 1, The Australian National University

Allele Symbol

Mtorchino

MGI Allele Accession ID

MGI:5484589

Ensembl Gene ID:

ENSMUSG00000028991

Transcript ID:

ENSMUST00000103221

Transcript name:

mtor-001

bp change:

T to G at position 705 on cDNA

Exon number:

Exon ID:

ENSMUSE00000524008

cDNA position:

705

Amino acid change:

Isoleucine to Serine at position 205

Sequence:
675	TTGTGGCTGTGTGGGACCCCAAGCAGGCCATCCGGGAAGGCGCTGTAGCGGCCCTTCGTGC	cDNA (forward strand)
195	F--V--A--V--W--D--P--K--Q--A--I--R--E--G--A--V--A--A--L--R--A	protein

Version:

NCBI m37, Ensembl build 67

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Low numbers of NK cells, few KLRG1hi expressing NK cells, low CD44 protein expression on CD4+ T cells in the blood

Phenotype Heterozygous State

Normal

Original Genetic Background

C57BL/6JAnu

Genetic Background Currently Maintained

C57BL/6JAnu

Strain identification

How is this strain characterised?

Genotyping

Suggested existing PCR names:

Amplifluor::4-147828770-igl101

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

lymphocyte
T cell
NK cell
ENU

Is the strain available in any form to other researchers

APB stock stored as:

Cryopreserved sperm (from 5 mice)

Are live mice available?

Unknown

MTA needs to be signed?

Yes