Home - Australian Phenome Bank

Mouse Strain information

APB ID

6900

Added

24/05/2012

Last Edited

09/06/2014

Strain nomenclature:

STOCK Tgfb2tm1Doe/MarpApb

Nickname:

TGFbeta2

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted knockout

ES Cell-Line Used

E14-1

Mode of Inheritance

Recessive

1. Affected Gene Name: transforming growth factor, beta 2

Chromosome	1
Affected Gene Symbol	Tgfb2
Protein expression of altered gene:	Nil
Genetic alteration of gene:	A 62 bp oligonucleotide from mouse TGFb2 exon 4 (Miller et al., 1989)was used to screen a genomic library derived from 129/J mouse DNA (Shull et al., 1992). A 4.7 kb SacI fragment spanning exons 4 through 6 was subcloned from the phage DNA and ligated into pBluescript(Strategene). A poly(A)- pMC1neo cassette (Strategene) was blunt end ligated into the BamHI site in exon 6. Next, the 3.5 kb targeting sequences containing 400 bp of 5¢ homology, the neo cassette, and approximately 2 kb of 3¢ homology were subcloned as a BamHI-SacI fragment into pBluescript.
Location on chromosome (bp):	188447065-188529871 bp, - strand (From NCBI annotation of NCBI Build 37)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:98726
Synonyms	Tgfb-2, Tgf-beta2
Allele Name	transforming growth factor, beta 2; targeted mutation 1, Thomas Doetschman
Allele Symbol	Tgfb2tm1Doe
MGI Allele Accession ID	MGI:1888389
Allele synonyms:	TGBbeta2-, Tgfb2-, TGF-beta2-

Mutant Construction Technique

None

Phenotype

Phenotype Heterozygous State

TGFb2-null mice exhibit perinatal mortality and a wide range of developmental defects for a single gene disruption. These include cardiac, lung, craniofacial, limb, spinal column, eye, inner ear and urogenital defects (Sanford et al., 1997.Development 124, 2659-2670). TGFb2 heterozygotes exhibit a 30% increase in nephron number (Sims-Lucas et al., Pediatr Res. 2008 Jun;63(6):607-12).

Original Genetic Background

129P2/OlaHsd

Genetic Background Currently Maintained

Black Swiss/129/Ola mixed

Strain identification

How is this strain characterised?

Genotyping

Suggested existing PCR names:

p5 (AATGTGCAGGATAATTGCTGC), p3 (AACTCCATAGATATGGGGATGC).

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Yes

Description of human condition:

Peters Anomaly

Applicable Research Areas

Developmental biology
Bone
Internal/organ
Cardiovascular

Keywords

signal transduction
kidney
heart

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 5 mice)

Are live mice available?

MTA needs to be signed?

Yes