Mouse Strain information
APB ID 7035
Added 03/08/2012
Last Edited 14/03/2025
Strain nomenclature: STOCK Rb1tm3Tyj Trp53tm1Brn Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/StvApb
Nickname: Osx-Cre p53fl/fl pRbfl/fl
Genetics
Strain Types Mutant strain
Genetic Details Transgenic
Targeted floxed
ES Cell-Line Used mixed
Mode of Inheritance Dominant
1.  Affected Gene Name: transformation related protein 53  
Chromosome 11
Affected Gene Symbol Trp53
Protein expression of altered gene: Nil
Genetic alteration of gene: Insertion of loxP sites flanking exons 2 through 10. No effect on the normal function of the gene.
Location on chromosome (bp): 69393861-69405375 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

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 MGI:98834
Synonyms p53
Allele Name transformation related protein 53; targeted mutation 1, Anton Berns
Allele Symbol Trp53tm1Brn
MGI Allele Accession ID MGI:1931011
Allele synonyms: p53Co, p53F, p53F2-10, p53F2-10F, p53Fl, p53Fl, p53flox, p53L, p53lox, p53LoxP, Tp53flx, Trp53F2-10, Trp53F2-F10, Trp53Fl, Trp53loxP
2.  Affected Gene Name: retinoblastoma 1  
Chromosome 14
Affected Gene Symbol Rb1
Protein expression of altered gene: Nil
Genetic alteration of gene: Single loxP sites flank exon 3.
Location on chromosome (bp): 73583480-73725629 bp, - strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

 MGI:97874
Synonyms pRb, Rb, Rb-1
Allele Name retinoblastoma 1; targeted mutation 3, Tyler Jacks
Allele Symbol Rb1tm3Tyj
MGI Allele Accession ID MGI:2450162
Allele synonyms: Rb12lox, Rbf, RbloxP
1.   Transgene: Osx-Cre:GFP - transgene insertion 1, Andrew P McMahon  
Point mutation sequence:
Insertion/Tagging details:
Guide(s) RNA sequence:
Mutant Construction Technique CRE recombinase-loxP technology
Phenotype
Phenotype Homozygous State Generate osteosarcoma (bone cancer) Conditional mouse osteosarcoma, dependent on p53 loss and potentiated by loss of Rb, mimics the human disease.
Phenotype Heterozygous State Slightly smaller, susceptible to malocclusion. Generate osteosarcoma if p53fl/+ but at long latency (1yr)
Original Genetic Background mixed (129 x C57BL/6 x FVB/N)
Genetic Background Currently Maintained C57BL/6
Coat Colour Black
Eye Colour Black
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Other Patent Technologies? yes
Associated IP rights? No
Does it Model a Human Condition? Yes
Description of human condition: Osteosarcoma
Research value of this Strain model of osteosarcoma faithful to the human disease, metastatic
Applicable Research Areas Bone
Cancer
Keywords
  • osteosarcoma
  • Osx-Cre
  • p53fl/fl
  • pRbfl/fl
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 3 mice)
Are live mice available? No
MTA needs to be signed? Yes


     

 


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