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Mouse Strain information

APB ID

7354

Added

19/06/2013

Last Edited

16/12/2022

Strain nomenclature:

B6.129-Foxo1tm1Flv Tg(Lck-cre)157Jxm/Anu

Nickname:

Lck-Cre:Foxo1-flox

Genetics

Strain Types

Mutant strain

Genetic Details

Transgenic: CRE
Targeted floxed

Mode of Inheritance

Recessive

1. Affected Gene Name: forkhead box O1

Chromosome	3
Affected Gene Symbol	Foxo1
Protein expression of altered gene:	Normal
Genetic alteration of gene:	A loxP site was inserted into the XbaI restriction site upstream of exon 1 and a frt-flanked neoymycin cassette with a 3' loxP site was inserted downstream of exon 1. Mice were crossed with transgenic Flipase to remove the neomycin cassette, leaving exon 1 floxed. Protein expression was unaffected as determined by immunoblot analysis of homozygote lymphocyte extracts.
Location on chromosome (bp):	52268337-52350109 bp, + strand From VEGA annotation of GRCm38
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:1890077
Synonyms	Afxh, FKHR, Fkhr1, Foxo1a
Allele Name	forkhead box O1; targeted mutation 1, Richard A Flavell
Allele Symbol	Foxo1tm1Flv
MGI Allele Accession ID	MGI:3840910
Allele synonyms:	Foxo1flox

1. Transgene: targeted mutation I57, Jamey Marth; transgene insertion I57, Jamey Marth

Point mutation sequence:

Insertion/Tagging details:

Guide(s) RNA sequence:

Mutant Construction Technique

None

Phenotype

Original Genetic Background

129

Genetic Background Currently Maintained

C57BL/6JAnu

Strain identification

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

T cell
transcription

Is the strain available in any form to other researchers

Possibly

APB stock stored as:

Cryopreserved sperm (from 3 mice)

Are live mice available?

Unknown

MTA needs to be signed?

Yes