Mouse Strain information
APB ID
7591
Added
14/02/2014
Last Edited
10/09/2014
Strain nomenclature:
C57BL/6-Gt(ROSA)26Sortm1(cre/ERT2)Tyj Recql4tm2272Arte Trp53tm1Brn /StvApb
Nickname:
R26-CreER Recql4fl/fl pRbfl/fl
Genetics
Strain Types
Mutant strain
Genetic Details
Targeted knockin
ES Cell-Line Used
C57Bl6
Mode of Inheritance
Dominant
1. Affected Gene Name:
transformation related protein 53
Chromosome
11
Affected Gene Symbol
Trp53
Protein expression of altered gene:
Nil
Genetic alteration of gene:
Insertion of loxP sites flanking exons 2 through 10. No effect on the normal function of the gene.
Location on chromosome (bp):
69393861-69405375 bp, + strand (From VEGA annotation of NCBI Build 37)
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
MGI:98834
Synonyms
p53
Allele Name
transformation related protein 53; targeted mutation 1, Anton Berns
Allele Symbol
Trp53tm1Brn
MGI Allele Accession ID
MGI:1931011
Allele synonyms:
p53Co, p53F, p53F2-10, p53F2-10F, p53Fl, p53Fl, p53flox, p53L, p53lox, p53LoxP, Tp53flx, Trp53F2-10, Trp53F2-F10, Trp53Fl, Trp53loxP
2. Affected Gene Name:
gene trap ROSA 26, Philippe Soriano
Chromosome
6
Affected Gene Symbol
Gt(ROSA)26Sor
Protein expression of altered gene:
Normal
Genetic alteration of gene:
A cassette containing Cre-ERT2 was inserted into the locus.
Location on chromosome (bp):
Chr6:113067428-113077333 bp, - strand Genetic Position: Chr6, 52.73 cM
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
MGI:104735
Synonyms
beta geo, Gtrgeo26, Gtrosa26, R26, ROSA26
Allele Name
gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Tyler Jacks
Allele Symbol
Gt(ROSA)26Sortm1(cre/ERT2)Tyj
MGI Allele Accession ID
MGI:3699244
Allele synonyms:
Cre-ERT2, Gt(ROSA)26Sortm1(cre/ESR1)Tyj, R26-Cre-ERT2, Rosa26CreER
3. Affected Gene Name:
RecQ protein-like 4
Chromosome
15
Affected Gene Symbol
Recql4
Protein expression of altered gene:
Reduced
Genetic alteration of gene:
LoxP placed around exon 9-10 of mouse Recql4
creates a true null allele when deleted
Location on chromosome (bp):
Chr15:76703553-76710559 bp, - strand
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
MGI:1931028
Synonyms
RECQ4
Allele Name
Recql4tm2272Arte
Allele Symbol
Recql4fl/fl
Allele synonyms:
Recql4 lox
Mutant Construction Technique
CRE recombinase-loxP technology
Phenotype
Phenotype Homozygous State
Normal in absence of tamoxifen treatment.
Phenotype Heterozygous State
Normal
Original Genetic Background
C57BL/6
Genetic Background Currently Maintained
C57BL/6
Coat Colour
Black
Eye Colour
Black
Strain identification
How is this strain characterised?
Genotyping
Suggested existing PCR names:
R26-CreER, p53fl/fl, Recql4fl/fl
Fertility and Strain maintenance
Fertility and Strain maintenance
Female
Male
Are homozygous mice viable?
Yes
Yes
Are homozygous mice fertile?
Yes
Yes
Are heterozygotes / hemizygotes* fertile?
Yes
Yes
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex
Generations Backcrossed
generated on a C57bl6 background
Mating Scheme
R26-CreERki/+ homozygous Recql4 and p53 intercrosses
Homozygous Matings Required?
No
Development Summary
Crossed the R26-CreER (purchased form Jackson Lab on a C57 background) to p53fl/fl Recql4fl/fl mice that were on a C57 background
Male Fertility Compromised?
No
Breeding Performance
Good
Special Husbandry Requirements
none
Relevant bibliographic / database references
Strain Specific References
Other Related References
1.
Ventura A, et al., Restoration of p53 function leads to tumour regression in vivo. Nature. 2007 Feb 8;445(7128):661-5
2.
Berman SD1, Calo E, Landman AS, Danielian PS, Miller ES, West JC, Fonhoue BD, Caron A, Bronson R, Bouxsein ML, Mukherjee S, Lees JA. Metastatic osteosarcoma induced by inactivation of Rb and p53 in the osteoblast lineage. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11851-6
General information
Other Patent Technologies?
yes
Associated IP rights?
Unknown
Does it Model a Human Condition?
Yes
Description of human condition:
Rothmund-Thomson syndrome
Research value of this Strain
can be used for conditional deletion of Recql4 and p53 in any cell.
Applicable Research Areas
Haematology
Keywords
R26-CreER
p53fl/fl
Recql4fl/fl
Is the strain available in any form to other researchers
Possibly
APB stock stored as:
Cryopreserved sperm (from 2 mice)
Are live mice available?
Limited
MTA needs to be signed?
Yes
