Mouse Strain information
APB ID 7753
Added 03/10/2014
Last Edited 12/10/2014
Strain nomenclature: BALB/c-Atp4btm1Ird/Apb
Nickname: Y20A; (kls/kls)
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
ES Cell-Line Used J1
Mode of Inheritance Recessive
1.  Affected Gene Name: ATPase, H+/K+ exchanging, beta polypeptide  
Chromosome 8
Affected Gene Symbol Atp4b
Protein expression of altered gene: Nil
Genetic alteration of gene: A neomycin selection casssette replaced 35 bp of exon 1. RT-PCR analysis demonstrated that no stable transcript was present in gastric tissue of homozygous mice, and western blot analysis showed that no stable protein was produced from this allele in gastric membranes derived from homozygous mice.
Location on chromosome (bp): Chr8:13386209-13396778 bp, - strand From NCBI annotation of GRCm38
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

 MGI:88114
Synonyms H+,K+-ATPase, H+/K+-ATPase beta, H,K-ATPase-Beta
Allele Name ATPase, H+/K+ exchanging, beta polypeptide; targeted mutation 1, Ian R van Driel
Allele Symbol Atp4btm1Ird
MGI Allele Accession ID MGI:2158258
Allele synonyms: Atp4bkls, Atp4bo, H/Kbeta, HKbeta-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State The stomachs of H+,K+-ATPase beta subunit-deficient mice were achlorhydric. Histological and immunocytochemical analyses with antibodies to the H+,K+-ATPase alpha subunit revealed that parietal cell development during ontogeny was retarded in H+,K+-ATPase beta subunit-deficient mice. In 15-day-old mice, cells with secretory canaliculi were observed in wild-type but not in H+,K+-ATPase beta subunit-deficient mice. Parietal cells of H+,K+-ATPase beta subunit-deficient mice 17 days and older contained an abnormal canaliculus that was dilated and contained fewer and shorter microvilli than normal. In older parietal cells, the abnormal canaliculus was massive (25 mu m in diameter) and contained few microvilli. We did not observe typical tubulovesicular membranes in any parietal cell from H+,K+-ATPase beta subunit-deficient mice. Histopathologic alterations were only observed in the stomach.
Phenotype Heterozygous State normal
Original Genetic Background C57BL/6 x 129
Genetic Background Currently Maintained BALB/c
Coat Colour white
Eye Colour red
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Other Patent Technologies? No
Associated IP rights? No
Does it Model a Human Condition? Unknown
Applicable Research Areas Gastrointestinal
Cell biology
Keywords
  • gastric acid
  • gastric proton pump
  • stomach
  • endocytosis
Is the strain available in any form to other researchers Possibly
APB stock stored as: Cryopreserved sperm (from 5 mice)
Are live mice available? No
MTA needs to be signed? No


     

 


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