Home - Australian Phenome Bank

Mouse Strain information

APB ID

8058

Added

22/06/2016

Last Edited

22/06/2016

Strain nomenclature:

B6(Cg).129-Mid1tm1Aash/AnuApb

Nickname:

Mid1 Fxy

Genetics

Strain Types

Mutant strain

Genetic Details

Targeted knockout
Targeted reporter

Mode of Inheritance

X-linked

1. Affected Gene Name: midline 1

Chromosome	X
Affected Gene Symbol	Mid1
Protein expression of altered gene:	Nil
Genetic alteration of gene:	Exon 2 was replaced with a lacZ and pgk-neo cassette via homologous recombination. LacZ reporter inserted at native ATG of Mid1 gene such that reporter expression reflects wildtype Mid1 expression. No Mid1 gene expression is detectable (ie. knockout).
Location on chromosome (bp):	169685199-169990798 bp, + strand From VEGA annotation of GRCm38
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:1100537
Synonyms	61B3-R, DXHXS1141, Fxy, Trim18
Allele Name	midline 1; targeted mutation 1, Alan Ashworth
Allele Symbol	Mid1tm1Aash
MGI Allele Accession ID	MGI:5562687

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Viable. Reduced / delayed fertility. Litter size may be slightly reduced. No other phenotype described as yet. Abnormal axon morphology: • axon length and branch number are increased in cultured cortical neurons • callosal axons grow further into the contralateral hemisphere at P4 • at P14, axon distribution in the corpus callosum is disturbed with a greater number of axon terminals, with a wider distribution in both the S1 and S2 regions • broader distribution of the contralateral axon projection pattern in the corpus callosum Embryogenesis phenotype: • no obvious midline defects, unlike in Opitz Syndrome patients

Phenotype Heterozygous State

Breeder pairs often only litter down after 3 months together, if they litter down at all. Around 1 in 3 produce litters.

Original Genetic Background

129

Genetic Background Currently Maintained

C57BL/6NCrlAnu

Strain identification

How is this strain characterised?

Genotyping

PCR protocols

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Yes

Description of human condition:

Opitz GBBB syndrome

Research value of this Strain

MID1 deficiency in humans causes Opitz GBBB syndrome. Most of the features of this disorder are not seen in these mice on the given backgrounds.

Applicable Research Areas

Reproduction
Models for human disease
Developmental biology
Cardiovascular

Keywords

X-linked
craniofacial
cleft lip

Additional Information

Strain re-animated from frozen stock of 4868, expanded and frozen down again.

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 5 mice)

APB stock genotyped verified by:

Unverified

Are live mice available?

Unknown

MTA needs to be signed?

Yes