A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. While RT-PCR analysis detects a high level of gene transcript in spleen and lymph nodes, and a lower level in lung, pancreas, thymus, bone marrow, skeletal muscle, stomach, small intestine, large intestine, cecum, testis, epididymis, seminal vesicle, coagulating gland and prostate gland, beta-galactosidase expression from this allele is detectable only in spleen and lymph nodes.
Location on chromosome (bp):
8236043-8257127 bp, + strand (From Ensembl annotation of GRCm38)
MGI Gene Accession ID
Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)
Transgenic mice carry two coinjected constructs containing rearranged mouse TCR alpha (Tcra-V2, Tcra-J(alpha26)) and TCR beta (Tcrb-V5, Tcrb-D(beta2), Tcrb-J(beta2.6)) chains. This transgenic T cell receptor recognizes ovalbumin residues 257-264 (SIINFEKL) in the context of H2Kb. The rearranged Tcra (alpha chain) cDNA specific for ovalbumin was isolated from T cells (clone AD10) and is expressed under the control of a 2-kb fragment of the H-2Kb promoter, a 700-bp fragment including the Ig H chain enhancer located 5' to the promoter, and a 1.7-kb BamHI-PstI fragment containing the 3' half of the human adult 8-globin gene (including part of exon 2, the exon 2-exon 3 C.F6 TCR a-chain gene that was truncated at the 3' XbaI site intron, exon 3) and polyadenylation signal. The Tcrb (beta chain) construct consists of a genomic fragment containing the Tcrb coding and endogenous promoter regions from a T cell clone (B3) specific for ovalbumin.
* Hemizygote as used here refers to males carring a mutation on the X chromosome or mice of either sex
carrying an inserted transgene with no homologous allele on the other chromosome.
