Home - Australian Phenome Bank

Mouse Strain information

APB ID

8660

Added

15/02/2019

Last Edited

15/02/2019

Strain nomenclature:

B6.Cg-Msh2tm1Ndw Rag1tm1Bal Samhd1em1Cjo/Cjo

Nickname:

SHMR

Genetics

Strain Types

Mutant strain

Genetic Details

CRISPR targeted
Targeted knockout

Mode of Inheritance

Recessive

1. Affected Gene Name: mutS homolog 2

Chromosome	17
Affected Gene Symbol	Msh2
Location on chromosome (bp):	87672557-87723713 bp, + strand (From NCBI annotation of GRCm38)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:101816

2. Affected Gene Name: recombination activating gene 1

Chromosome	2
Affected Gene Symbol	Rag1
Genetic alteration of gene:	The gene was disrupted by insertion of a PGK-neo cassette into the coding region at amino acid 330 resulting in a frameshift mutation.
Location on chromosome (bp):	101638282-101649501 bp, - strand (From Ensembl annotation of GRCm38)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:97848
Synonyms	Rag-1
Allele Name	recombination activating gene 1; targeted mutation 1, David Baltimore
Allele Symbol	Rag1tm1Bal
MGI Allele Accession ID	MGI:2448994
Allele synonyms:	Rag-1-

3. Affected Gene Name: SAM domain and HD domain, 1

Chromosome	2
Affected Gene Symbol	Samhd1
Genetic alteration of gene:	CRISPR/CAs9 mediated mutation: 25bp deletion in exon1
Location on chromosome (bp):	157097533-157135265 bp, - strand (From Ensembl annotation of GRCm38)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:1927468
Synonyms	E330031J07Rik

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Unknown

Original Genetic Background

Mixed

Genetic Background Currently Maintained

C57BL/6

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 5 mice)

Are live mice available?

Unknown

MTA needs to be signed?

Yes