Mouse Strain information
APB ID 8797
Added 01/10/2019
Last Edited 04/10/2019
Strain nomenclature: B6.129S7-Spp1tm1Rit/Apb
Nickname: Osteopontin null
Genetics
Strain Types Mutant strain
Genetic Details Targeted knockout
Mode of Inheritance Recessive
1.  Affected Gene Name: secreted phosphoprotein 1  
Chromosome 5
Affected Gene Symbol Spp1
Genetic alteration of gene: Insertion of a neomycin resistance cassette into exon 6 immediately 5' of the RGD sequence disrupted the Spp1 gene. Northern analysis of homozygous mutant kidney detected low levels of a high molecular weight RNA species, but no wild-type or truncated Spp1 transcript. Immunoblot analysis detected low levels of a truncated form of the protein in bone but not in other tissues, fluids, or cells of homozygous mutant mice.
Location on chromosome (bp): 104435118-104441050 bp, + strand (From Ensembl annotation of GRCm38)
MGI Gene Accession ID

Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)

 MGI:98389
Synonyms 2ar, 44kDa bone phosphoprotein, Apl-1, BNSP, bone sialoprotein 1, ETA-1, minopontin, OP, Opn, Opnl, osteopontin, osteopontin-like protein, Ric, Spp-1
Allele Name secreted phosphoprotein 1; targeted mutation 1, Susan R Rittling
Allele Symbol Spp1tm1Rit
MGI Allele Accession ID MGI:1932084
Allele synonyms: Eta-1-, Opn-
Mutant Construction Technique None
Phenotype
Phenotype Homozygous State Mortality/aging: N • homozygotes are viable, fertile and overtly normal with no significant differences in lactation, growth development, or organ and tissue histology Decreased osteoclast cell number: • fewer osteoclasts accumulate at the site of an implanted bone disc compared to in similarly treated wild-type mice. Increased osteoclast cell number: • homozygotes exhibit a 3-fold increase in osteoclast number relative to wild-type mice; ovariectomy does not increase the number of osteoclasts significantly • in vitro, several-fold more osteoclasts are generated in co-culture systems with wild-type calvarial osteoblast cells by using spleen cells (3- to 13-fold) or bone marrow cells (~2- to 4-fold) from homozygous mutant mice compared with wild-type mice. Abnormal osteoclast physiology: • in contrast to wild-type mice, homozygotes are resistant to ovariectomy-induced bone resorption despite a comparable reduction in uterine weight (25-30%) • ovariectomized homozygotes exhibit a 12% reduction in trabecular bone volume in the absence of increased bone formation, whereas ovariectomized wild-type mice show a 58% reduction in trabecular bone volume along with a 73% increase in bone formation • bone resorption and osteoclast formation induced by PTH in cultured mouse calvariae are absent in calvariae from homozygous mutant mice Decreased interferon-gamma secretion: • following treatment with polyvinyl pyrrolidone, mice exhibit a 90% reduction in IL12 production compared with in similarly treated wild-type mice • splenocytes from L. monocytogenes infected mice produce less IFN-gamma when restimulated with heat-killed bacteria compared with similarly treated wild-type mice Increased interleukin-10 secretion: • draining lymph node cells from HSV-1-infected mice produce increased IL10 compared with cells from similarly treated wild-type mice. Decreased interleukin-12 secretion: • following treatment with polyvinyl pyrrolidone, mice exhibit a 95% reduction in IL12 production compared with in similarly treated wild-type mice • draining lymph node cells and splenocytes from HSV-1-infected mice produce decreased IL12 compared with cells from similarly treated wild-type mice. Increased interleukin-4 secretion: • draining lymph node cells from HSV-1-infected mice produce increased IL4 compared with cells from similarly treated wild-type mice. Abnormal inflammatory response: • mice treated with polyvinyl pyrrolidone fail to exhibit a granulomatous response unlike similarly treated wild-type mice Decreased susceptibility to type IV hypersensitivity reaction: • mice infected with HSV-1 do not display a tuberculin-type delayed-type hypersensitivity when challenged with HSV-1 unlike similarly treated wild-type mice Abnormal response to infection: • clearance of Listeria monocytogenes is defective compared to in similarly treated wild-type mice • splenocytes from L. monocytogenes infected mice produce less IFN-gamma when restimulated with heat-killed bacteria compared with similarly treated wild-type mice Decreased susceptibility to viral infection: • mice infected with HSV-1 do not display a tuberculin-type delayed-type hypersensitivity when challenged with HSV-1 unlike similarly treated wild-type mice • corneal HSV-1 infection fails to trigger herpes simplex keratitis unlike in similarly treated wild-type mice
Phenotype Heterozygous State Normal
Original Genetic Background 129S7/SvEvBrd
Genetic Background Currently Maintained C57BL/6
Strain identification
How is this strain characterised? Genotyping
Fertility and Strain maintenance
Fertility and Strain maintenance
Relevant bibliographic / database references
Strain Specific References
Other Related References
General information
Associated IP rights? Unknown
Does it Model a Human Condition? Unknown
Applicable Research Areas Bone
Is the strain available in any form to other researchers Yes
APB stock stored as: Cryopreserved sperm (from 3 mice)
Are live mice available? Unknown
MTA needs to be signed? Yes


     

 


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