Home - Australian Phenome Bank

Mouse Strain information

APB ID

8915

Added

23/03/2020

Last Edited

13/04/2020

Strain nomenclature:

B6(Cg)-Ptprca Rag1tm1Mom Ightm1Rbr Tg(IgkHyHEL10)1Rbr/AnuApb

Nickname:

SwHel:RAG1KO:Hc.Lc:Ly5a

Genetics

Strain Types

Mutant strain
Congenic

Genetic Details

Transgenic
Targeted knockin
Congenic

Mode of Inheritance

Recessive

1. Affected Gene Name: recombination activating 1

Chromosome	2
Affected Gene Symbol	Rag1
Genetic alteration of gene:	A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice.
Location on chromosome (bp):	101638282-101649501 bp, - strand (From Ensembl annotation of GRCm38)
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:97848
Synonyms	Rag-1
Allele Name	recombination activating 1; targeted mutation 1, Peter Mombaerts
Allele Symbol	Rag1tm1Mom
MGI Allele Accession ID	MGI:1857241
Allele synonyms:	Rag-, Rag1-, RAG-1-, Rag-1KO, RAG1null, Rag1tm1Mom

2. Affected Gene Name: immunoglobulin heavy chain complex

Chromosome	12
Affected Gene Symbol	Igh
Genetic alteration of gene:	The HyHEL10 hybridoma produces an antibody that binds hen egg lysozyme peptide (HEL) with high affinity. The rearranged variable heavy gene segment from the HyHEL10 hybridoma replaced the endogenous J gene segments of the IgH locus. A floxed neomycin cassette was placed upstream for targeting purposes. About 0.4% of B cells produce antibody that bind HEL with high affinity.
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:96442
Allele Name	immunoglobulin heavy chain complex; targeted mutation 1, Robert Brink
Allele Symbol	Ightm1Rbr
MGI Allele Accession ID	MGI:3800383
Allele synonyms:	VH-10tar

1. Transgene: transgene insertion 1, Robert Brink

1. Affected Congenic Gene Name: protein tyrosine phosphatase, receptor type, C

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

Rag1 KO mice lack mature T and B cells. Homozygous for SwHEL Heavy chain (Hc/Hc) Heterozygous for SwHEL Light chain (Lc/WT), B cell specific for Hen egg lysozyme.

Phenotype Heterozygous State

All B cells are specific for Hen egg lysozyme

Original Genetic Background

C57BL/6

Genetic Background Currently Maintained

C57BL/6

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

B cell
Hen Egg Lysozyme (HEL)

Is the strain available in any form to other researchers

Yes

APB stock stored as:

Cryopreserved sperm (from 2 mice)

Are live mice available?

Unknown

MTA needs to be signed?

Yes