Home - Australian Phenome Bank

Mouse Strain information

APB ID

9041

Added

14/07/2020

Last Edited

28/08/2020

Strain nomenclature:

Atmintm1.2Jhh Cd79atm1(cre)Reth Dynll1tm1.1Jhh

Nickname:

Ascizfl Mb-1Cre Dynll1fl

Genetics

Strain Types

Inbred

Genetic Details

Targeted knockout

Mode of Inheritance

Recessive

1. Affected Gene Name: ATM interactor

Chromosome	8
Affected Gene Symbol	Atmin
Protein expression of altered gene:	Reduced
Genetic alteration of gene:	A loxP site was inserted upstream of exon D, and an FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon D. Flp-mediated recombination removed the neo cassette and left exon D floxed.
Location on chromosome (bp):	116943393-116960445 bp
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:2682328
Synonyms	Asciz, gpg6, MGC:79206, mKIAA0431
Allele Name	ATM interactor; targeted mutation 1.2, Jorg Heierhorst
Allele Symbol	Atmintm1.2Jhh
MGI Allele Accession ID	MGI:5463937
Allele synonyms:	Ascizfl

2. Affected Gene Name: Dynein light chain LC8-type 1

Chromosome	5
Affected Gene Symbol	Dynll1
Genetic alteration of gene:	The promoter, exon 1 and exon 2 were floxed. Flp-mediated recombination removed the puromycin resistance cassette and left and FRT site upstream of the second loxP site in intron 2.
Location on chromosome (bp):	115297110-115300990 bp
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:1861457
Synonyms	8kDa LC, DLC8, Dnclc1, Pin
Allele Name	Dynein light chain LC8-type 1; targeted mutation 1.1, Jorg Heierhorst
Allele Symbol	Dynll1tm1.1Jhh
MGI Allele Accession ID	MGI:5755433
Allele synonyms:	Dynll1fl

1. Transgene: CD79A antigen (immunoglobulin-associated alpha); targeted mutation 1, Michael Reth

Mutant Construction Technique

CRE recombinase-loxP technology

Phenotype

Phenotype Homozygous State

Severe reduction of B cell numbers (in Asciz f/f Mb1-Cre Ki/+ Dynll1 f/f conditional cKO mice)

Phenotype Heterozygous State

Normal

Genetic Background Currently Maintained

C57BL/6

Strain Images

Photographs of representative spleens from Cd79atm1(cre)Reth/Cd79a+ (Control Cre), Atmintm1.2Jhh/Atmintm1.2Jhh (Control flox), and an Atmintm1.2Jhh/Atmintm1.2Jhh/ Cd79atm1(cre)Reth/Cd79a+ deleter.

Generation of the conditional Atmintm1.2Jhh (flox) allele. (A) Atmin (ASCIZ) domain organization and conditional deletion strategy. The probe and fragment sizes for Southern blot analysis and primer locations (F1, R1, and R2) and product sizes for PCR genotyping are indicated. SCD, SQ/TQ cluster domain; TAD, transcription activation domain; ZF, zinc finger.

Strain identification

How is this strain characterised?

Genotyping

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

Strain Specific References

Other Related References

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Applicable Research Areas

Immunology and inflammation

Keywords

B cell development
Adaptive and innate B cells
Lymphoid cells

Is the strain available in any form to other researchers

Possibly

APB stock stored as:

Cryopreserved sperm (from 5 mice)

Are live mice available?

Unknown

MTA needs to be signed?

Yes