Home - Australian Phenome Bank

Mouse Strain information

APB ID

9212

Added

07/07/2021

Last Edited

12/02/2025

Strain nomenclature:

C57Bl/6N-Bsnem2Anu70/Apb

Nickname:

Bsn KO - ASD1106:BsnemL1903M:70

Genetics

Strain Types

Inbred

Genetic Details

CRISPR targeted

Mode of Inheritance

Semi-dominant

1. Affected Gene Name: bassoon

Chromosome	9
Affected Gene Symbol	Bsn
Genetic alteration of gene:	Bsn KO, founder mouse Targeting mouse Bsn exon 5 at genomic position 107,990,045 (ENSMUSE00000469753) on Chr: 9 (GRCm39 genome assembly) by direct pronuclear injections using CRISPR/Cas9 complexed with ssODN donor to change amino acid from Leucine (CTG) to Methionine (ATG).
Location on chromosome (bp):	107973221-108067583 bp
MGI Gene Accession ID Please also use this link to determine if other mutants have been registered with MGI (Mouse Genome Informatics)	MGI:1277955
Synonyms	presynaptic cytomatrix protein
Allele Name	Bsn:endonuclease-mediated mutation 2, Australian National University
Allele Symbol	Bsnem2Anu
Ensembl Gene ID:	ENSMUSG00000032589
Transcript ID:	ENSMUST00000035208

CRISPR types

Knockout

Knockout details:

targeting Bsn exon 5, Leucine at position 1903 was changed to Methionine.

Point mutation sequence:

Insertion/Tagging details:

Guide(s) RNA sequence:

GAAGAGCCGGCAGGTGCCCTGGACCTCACCGGAATGAGGCCCGAGAGCCAGaTGGCATGCTGTGACATGGTCTACAAGTTTCCTTTTGGCAG Guide sequence: CCATGTCACAGCATGCCAGC TGG

Mutant Construction Technique

None

Phenotype

Phenotype Homozygous State

unknown

Phenotype Heterozygous State

unknown

Genetic Background Currently Maintained

C57BL/6NCrl

Strain identification

Fertility and Strain maintenance

Fertility and Strain maintenance

Relevant bibliographic / database references

General information

Associated IP rights?

Unknown

Does it Model a Human Condition?

Unknown

Is the strain available in any form to other researchers

Possibly

APB stock stored as:

Cryopreserved sperm (from 1 mice)

Are live mice available?

Unknown

MTA needs to be signed?

Yes